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Mouse Anti-MAFB (AA 32-320 ) Recombinant Antibody (CBFYM-1280) (CBMAB-M1439-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1280
Antibody Isotype
IgG2b
Application
WB, IHC, FC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA
Preservative
0.02% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 32-320

Target

Full Name
MAF bZIP transcription factor B
Introduction
The protein encoded by this gene is a basic leucine zipper transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.
Entrez Gene ID
9935
UniProt ID
Q9Y5Q3
Alternative Names
MAF BZIP Transcription Factor B; V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog B; KRML; MAFB/Kreisler Basic Region/Leucine Zipper Transcription Factor; V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B; Kreisler (Mouse) Maf-Related Leucine Zipper Homolog
Function
Acts as a transcriptional activator or repressor (PubMed:27181683).
Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity).
Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).
Biological Process
Abducens nerve formationISS:UniProtKB
Brain segmentationIEA:Ensembl
Cornified envelope assemblyIEA:Ensembl
Fat cell differentiationISS:UniProtKB
Inner ear morphogenesisIEA:Ensembl
Integrated stress response signaling1 PublicationIC:ComplexPortal
Keratinocyte differentiationIEA:Ensembl
Negative regulation of erythrocyte differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of osteoclast differentiationISS:UniProtKB
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Protein processingIEA:Ensembl
Regulation of myeloid cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Respiratory gaseous exchange by respiratory systemIEA:Ensembl
Rhombomere 5 developmentIEA:Ensembl
Rhombomere 6 developmentIEA:Ensembl
Segment specificationIEA:Ensembl
Sensory organ developmentManual Assertion Based On ExperimentTAS:ProtInc
T cell differentiation in thymusIEA:Ensembl
Thymus developmentIEA:Ensembl
Cellular Location
Nucleus
Involvement in disease
Multicentric carpotarsal osteolysis syndrome (MCTO):
A rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
Duane retraction syndrome 3 with or without deafness (DURS3):
A form of Duane retraction syndrome, a congenital eye movement disorder characterized by a failure of cranial nerve VI (the abducens nerve) to develop normally, resulting in restriction or absence of abduction, adduction or both, narrowing of the palpebral fissure, and retraction of the globe on attempted adduction. Undiagnosed in children, it can lead to amblyopia, a permanent uncorrectable loss of vision. Some DURS3 patients manifest sensorineural hearing loss.
PTM
Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors (By similarity).
More Infomation

Fujino, M., Ojima, M., & Takahashi, S. (2023). Exploring Large MAF Transcription Factors: Functions, Pathology, and Mouse Models with Point Mutations. Genes, 14(10), 1883.

Samir, O., Kobayashi, N., Nishino, T., Siyam, M., Yadav, M. K., Inoue, Y., ... & Hamada, M. (2022). Transcription factor MAFB as a prognostic biomarker for the lung adenocarcinoma. International Journal of Molecular Sciences, 23(17), 9945.

Sun, Y., Liu, T., & Bai, W. (2022). MAF bZIP Transcription Factor B (MAFB) Protected Against Ovalbumin-Induced Allergic Rhinitis via the Alleviation of Inflammation by Restoring the T Helper (Th) 1/Th2/Th17 Imbalance and Epithelial Barrier Dysfunction. Journal of Asthma and Allergy, 267-280.

Saiga, H., Ueno, M., Tanaka, T., Kaisho, T., & Hoshino, K. (2022). Transcription factor MafB-mediated inhibition of type I interferons in plasmacytoid dendritic cells. International Immunology, 34(3), 159-172.

He, L., Gao, M., Pratt, H., Weng, Z., & Struhl, K. (2022). MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles. Plos one, 17(4), e0264799.

Takahashi, S. (2021). Functional analysis of large MAF transcription factors and elucidation of their relationships with human diseases. Experimental Animals, 70(3), 264-271.

Russell, R., Carnese, P. P., Hennings, T. G., Walker, E. M., Russ, H. A., Liu, J. S., ... & Hebrok, M. (2020). Loss of the transcription factor MAFB limits β-cell derivation from human PSCs. Nature communications, 11(1), 2742.

Hörberg, J., & Reymer, A. (2019). BZip transcription factors modulate DNA supercoiling transitions. bioRxiv, 2019-12.

Tang, G. H., Xiong, Y., Liu, Y., Song, Z. H., Yang, Y., Shen, G. M., ... & Jiang, H. B. (2019). The Transcription factor mafB regulates the susceptibility of Bactrocera dorsalis to abamectin via GSTz2. Frontiers in Physiology, 10, 1068.

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For research use only. Not intended for any clinical use.

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