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Mouse Anti-KRT18 Recombinant Antibody (4D11E4) (CBMAB-K1698-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4D11E4
Antibody Isotype
IgG2b
Application
WB, IHC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Keratin 18
Introduction
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
3875
UniProt ID
P05783
Alternative Names
Keratin 18; Cell Proliferation-Inducing Gene 46 Protein; Keratin 18; Type I; CK-18; CYK18; K18;
Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity).
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Cell cycleIEA:UniProtKB-KW
Extrinsic apoptotic signaling pathwayIEA:Ensembl
Golgi to plasma membrane CFTR protein transportManual Assertion Based On ExperimentIDA:UniProtKB
Hepatocyte apoptotic processIEA:Ensembl
Intermediate filament cytoskeleton organizationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Tumor necrosis factor-mediated signaling pathwayIEA:Ensembl
Cellular Location
Cytoplasm, perinuclear region
Nucleus, nucleolus
Involvement in disease
Cirrhosis (CIRRH):
A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension.
PTM
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
More Infomation

Cui, J., Ma, Q., Zhang, C., Li, Y., Liu, J., Xie, K., ... & Tang, C. (2023). Keratin 18 Depletion as a Possible Mechanism for the Induction of Apoptosis and Ferroptosis in the Rat Hippocampus After Hypobaric Hypoxia. Neuroscience, 513, 64-75.

Wang, P. B., Chen, Y., Ding, G. R., Du, H. W., & Fan, H. Y. (2021). Keratin 18 induces proliferation, migration, and invasion in gastric cancer via the MAPK signalling pathway. Clinical and Experimental Pharmacology and Physiology, 48(1), 147-156.

Yin, S., Song, M., Zhao, R., Liu, X., Kang, W. K., Lee, J. M., ... & Lee, M. H. (2020). Xanthohumol inhibits the growth of keratin 18-overexpressed esophageal squamous cell carcinoma in vitro and in vivo. Frontiers in cell and developmental biology, 8, 366.

Muriel, J. M., O’Neill, A., Kerr, J. P., Kleinhans-Welte, E., Lovering, R. M., & Bloch, R. J. (2020). Keratin 18 is an integral part of the intermediate filament network in murine skeletal muscle. American Journal of Physiology-Cell Physiology, 318(1), C215-C224.

Sirniö, P., Väyrynen, J. P., Mutt, S. J., Herzig, K. H., Walkowiak, J., Klintrup, K., ... & Tuomisto, A. (2020). Systemic inflammation is associated with circulating cell death released keratin 18 fragments in colorectal cancer. Oncoimmunology, 9(1), 1783046.

Golob-Schwarzl, N., Bettermann, K., Mehta, A. K., Kessler, S. M., Unterluggauer, J., Krassnig, S., ... & Haybaeck, J. (2019). High keratin 8/18 ratio predicts aggressive hepatocellular cancer phenotype. Translational oncology, 12(2), 256-268.

Zhang, J., Hu, S., & Li, Y. (2019). KRT18 is correlated with the malignant status and acts as an oncogene in colorectal cancer. Bioscience reports, 39(8), BSR20190884.

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For research use only. Not intended for any clinical use.

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