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Mouse Anti-KIF5B Recombinant Antibody (KN-02) (CBMAB-K0080-LY)


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Summary

Host Animal
Mouse
Specificity
Pig, Human, Mouse, Rat
Clone
KN-02
Antibody Isotype
IgM
Application
ICC/IF

Basic Information

Immunogen
Enriched fraction of porcine brain kinesin
Specificity
Pig, Human, Mouse, Rat
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
kinesin family member 5B
Introduction
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726).
Entrez Gene ID
Human3799
Mouse16573
Rat117550
Pig595132
UniProt ID
HumanP33176
MouseQ61768
RatQ2PQA9
PigF1RWC5
Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity).
Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726).
Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).
Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed:24088571).
Biological Process
Anterograde axonal protein transportISS:UniProtKB
Anterograde dendritic transport of neurotransmitter receptor complexManual Assertion Based On ExperimentIBA:GO_Central
Anterograde neuronal dense core vesicle transportISS:ARUK-UCL
Axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to interferon-gammaIEA:Ensembl
Centrosome localizationManual Assertion Based On ExperimentIMP:UniProtKB
Cytoplasm organizationIEA:Ensembl
Cytoskeleton-dependent intracellular transportManual Assertion Based On ExperimentIBA:GO_Central
Lysosome localizationManual Assertion Based On ExperimentIMP:UniProtKB
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIMP:UniProtKB
Plus-end-directed vesicle transport along microtubuleIEA:Ensembl
Positive regulation of potassium ion transportManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of synaptic transmission, GABAergicIEA:Ensembl
Regulation of membrane potentialManual Assertion Based On ExperimentIDA:BHF-UCL
Retrograde neuronal dense core vesicle transportISS:ARUK-UCL
Stress granule disassemblyISS:BHF-UCL
Synaptic vesicle transportManual Assertion Based On ExperimentIBA:GO_Central
Vesicle transport along microtubuleManual Assertion Based On ExperimentIDA:ARUK-UCL
Cellular Location
Cytoplasm, cytoskeleton; Cytolytic granule membrane; Lysosome membrane. Uniformly distributed between soma and neurites in hippocampal neurons.
More Infomation

Flex, E., Albadri, S., Radio, F. C., Cecchetti, S., Lauri, A., Priolo, M., ... & Tartaglia, M. (2023). Dominantly acting KIF5B variants with pleiotropic cellular consequences cause variable clinical phenotypes. Human Molecular Genetics, 32(3), 473-488.

Charles Jacob, H. K., Signorelli, R., Charles Richard, J. L., Kashuv, T., Lavania, S., Middleton, A., ... & Saluja, A. K. (2022). Identification of novel early pancreatic cancer biomarkers KIF5B and SFRP2 from “first contact” interactions in the tumor microenvironment. Journal of experimental & clinical cancer research, 41(1), 1-22.

Kuroda, N., Trpkov, K., Gao, Y., Tretiakova, M., Liu, Y. J., Ulamec, M., ... & Hes, O. (2020). ALK rearranged renal cell carcinoma (ALK-RCC): a multi-institutional study of twelve cases with identification of novel partner genes CLIP1, KIF5B and KIAA1217. Modern Pathology, 33(12), 2564-2579.

Serra-Marques, A., Martin, M., Katrukha, E. A., Grigoriev, I., Peeters, C. A., Liu, Q., ... & Akhmanova, A. (2020). Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends. Elife, 9, e61302.

Zhao, J., Fok, A. H. K., Fan, R., Kwan, P. Y., Chan, H. L., Lo, L. H. Y., ... & Lai, K. O. (2020). Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. elife, 9, e53456.

Gan, H., Xue, W., Gao, Y., Zhu, G., Chan, D., Cheah, K. S., & Huang, J. (2019). KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes. Cell & Bioscience, 9, 1-16.

Moamer, A., Hachim, I. Y., Binothman, N., Wang, N., Lebrun, J. J., & Ali, S. (2019). A role for kinesin-1 subunits KIF5B/KLC1 in regulating epithelial mesenchymal plasticity in breast tumorigenesis. EBioMedicine, 45, 92-107.

Chang, K. T. E., Tay, A. Z. E., Kuick, C. H., Chen, H., Algar, E., Taubenheim, N., ... & Chow, C. W. (2019). ALK-positive histiocytosis: an expanded clinicopathologic spectrum and frequent presence of KIF5B-ALK fusion. Modern Pathology, 32(5), 598-608.

Ni, Y. X., Zhou, N., Xue, W. Q., Rong, L., Yung, W. H., Lin, R. Z., ... & Huang, J. D. (2018). A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating. Cell Discovery, 4(1), 65.

Gow, C. H., Liu, Y. N., Li, H. Y., Hsieh, M. S., Chang, S. H., Luo, S. C., ... & Shih, J. Y. (2018). Oncogenic function of a KIF5B-MET fusion variant in non-small cell lung cancer. Neoplasia, 20(8), 838-847.

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For research use only. Not intended for any clinical use.

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