Mouse Anti-IPO7 Recombinant Antibody (4G6) (CBMAB-R0553-CN)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4G6

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa. 950-1038 from IPO7 (NP_006382) with GST tag

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Target

Full Name

importin 7

Introduction

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq, Jul 2008]

Entrez Gene ID

10527

UniProt ID

O95373

Alternative Names

Importin 7

Function

Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones.
(Microbial infection) Mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. Binds and mediates the nuclear import of HIV-1 Rev.

Biological Process

Innate immune responseIEA:Ensembl
Negative regulation of cyclin-dependent protein serine/threonine kinase activityManual Assertion Based On ExperimentTAS:ARUK-UCL
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Nucleus

More Infomation

References

Xiao, Z., Zhang, Y., Yuan, G., & Yang, G. (2023). Reactive Oxygen Species-Induced Inhibition of Odontoblastic Differentiation of Mouse Dental Papilla Cells Is Mediated by Downregulation of Importin 7. Stem Cells and Development, 32(9-10), 258-269.

Itoh, Y., Tokunaga, M., Miyamoto, Y., Suzuki, T., Takada, A., Ninomiya, A., ... & Okamoto, T. (2023). Importin-7-dependent nuclear localization of the Flavivirus core protein is required for infectious virus production. bioRxiv, 2023-10.

Zhang, Y., Zhang, H., Xiao, Z., Yuan, G., & Yang, G. (2022). IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation. Stem Cells, 40(11), 1020-1030.

Chen, J., Hu, Y., Teng, Y., & Yang, B. (2021). Increased nuclear transporter importin 7 contributes to the tumor growth and correlates with CD8 T cell infiltration in cervical cancer. Frontiers in Cell and Developmental Biology, 9, 732786.

Yang, Y. C., & Sugden, B. (2021). Epstein-Barr Virus Limits the Accumulation of IPO7, an Essential Gene Product. Frontiers in Microbiology, 12, 643327.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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