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Rabbit Anti-INSR (phospho Y1185) Recombinant Antibody (BA0057) (CBMAB-0391CQ)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
BA0057
Antibody Isotype
IgG
Application
ICC, IP, WB

Basic Information

Immunogen
Synthetic phosphopeptide from residues surrounding Tyrosine 1185 of the human Insulin Receptor
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
Insulin receptor is a tetramer of 2 alpha and 2 beta subunits. The alpha and beta subunits are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. The INSR gene spans more than 120 kb and has 22 exons. The 11 exons encoding the alpha subunit are dispersed over more than 90 kb, whereas the 11 exons encoding the beta subunit are located together in a region of about 30 kb. Three transcriptional initiation sites were identified, located 276, 282, and 283 bp upstream of the translation initiation site. This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
3643
UniProt ID
P06213
Alternative Names
HHF5; CD220
More Infomation

Iraburu, M. J., Garner, T., & Montiel-Duarte, C. (2021). Revising endosomal trafficking under insulin receptor activation. International Journal of Molecular Sciences, 22(13), 6978.

Arcidiacono, B., Chiefari, E., Foryst-Ludwig, A., Curro, G., Navarra, G., Brunetti, F. S., ... & Brunetti, A. (2020). Obesity-related hypoxia via miR-128 decreases insulin-receptor expression in human and mouse adipose tissue promoting systemic insulin resistance. EBioMedicine, 59.

Zhou, B., Liu, J., Zeng, L., Zhu, S., Wang, H., Billiar, T. R., ... & Kang, R. (2020). Extracellular SQSTM1 mediates bacterial septic death in mice through insulin receptor signalling. Nature microbiology, 5(12), 1576-1587.

Fernández-de Frutos, M., Galán-Chilet, I., Goedeke, L., Kim, B., Pardo-Marqués, V., Pérez-García, A., ... & Ramírez, C. M. (2019). MicroRNA 7 Impairs Insulin Signaling and Regulates A β Levels through Posttranscriptional Regulation of the Insulin Receptor Substrate 2, Insulin Receptor, Insulin-Degrading Enzyme, and Liver X Receptor Pathway. Molecular and Cellular Biology, 39(22), e00170-19.

Cignarelli, A., Genchi, V. A., Perrini, S., Natalicchio, A., Laviola, L., & Giorgino, F. (2019). Insulin and insulin receptors in adipose tissue development. International journal of molecular sciences, 20(3), 759.

Payankaulam, S., Raicu, A. M., & Arnosti, D. N. (2019). Transcriptional regulation of INSR, the insulin receptor gene. Genes, 10(12), 984.

Chen, Y., Huang, L., Qi, X., & Chen, C. (2019). Insulin receptor trafficking: consequences for insulin sensitivity and diabetes. International journal of molecular sciences, 20(20), 5007.

Tsai, S., Clemente-Casares, X., Zhou, A. C., Lei, H., Ahn, J. J., Chan, Y. T., ... & Winer, D. A. (2018). Insulin receptor-mediated stimulation boosts T cell immunity during inflammation and infection. Cell metabolism, 28(6), 922-934.

Hersom, M., Helms, H. C., Schmalz, C., Pedersen, T. Å., Buckley, S. T., & Brodin, B. (2018). The insulin receptor is expressed and functional in cultured blood-brain barrier endothelial cells but does not mediate insulin entry from blood to brain. American Journal of Physiology-Endocrinology and Metabolism, 315(4), E531-E542.

Yang, B. Y., Zhai, G., Gong, Y. L., Su, J. Z., Peng, X. Y., Shang, G. H., ... & Xie, S. Q. (2018). Different physiological roles of insulin receptors in mediating nutrient metabolism in zebrafish. American Journal of Physiology-Endocrinology and Metabolism, 315(1), E38-E51.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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