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Mouse Anti-ILK Recombinant Antibody (4F10) (CBMAB-A4451-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4F10
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ILK (AAH01554, 341 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
integrin-linked kinase
Introduction
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Entrez Gene ID
Human3611
Mouse16202
Rat170922
UniProt ID
HumanQ13418
MouseO55222
RatQ99J82
Alternative Names
DKFZp686F1765; P59
Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715).
May act as a mediator of inside-out integrin signaling (PubMed:10712922).
Focal adhesion protein part of the complex ILK-PINCH (PubMed:10712922).
This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway (PubMed:10712922).
Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells (PubMed:10712922).
Regulates cell motility by forming a complex with PARVB (PubMed:32528174).
Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
Biological Process
Branching involved in ureteric bud morphogenesisIEA:Ensembl
Cell morphogenesisManual Assertion Based On ExperimentIMP:ARUK-UCL
Cell projection organizationIEA:Ensembl
Cell-matrix adhesionManual Assertion Based On ExperimentIBA:GO_Central
Establishment or maintenance of epithelial cell apical/basal polarityIEA:Ensembl
Fibroblast migrationIEA:Ensembl
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:MGI
Myelination in peripheral nervous systemIEA:Ensembl
Negative regulation of neural precursor cell proliferationIEA:Ensembl
Nerve developmentIEA:Ensembl
Outflow tract morphogenesisIEA:Ensembl
Peptidyl-serine phosphorylationIEA:Ensembl
Platelet aggregationManual Assertion Based On ExperimentHMP:UniProtKB
Positive regulation of BMP signaling pathwayIEA:Ensembl
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of cell population proliferationIEA:Ensembl
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIMP:CAFA
Positive regulation of osteoblast differentiationIEA:Ensembl
Positive regulation of phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIGI:ARUK-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Protein kinase B signalingIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:UniProtKB
Tumor necrosis factor-mediated signaling pathwayManual Assertion Based On ExperimentIMP:CAFA
Cellular Location
Cell membrane; Cytoplasm, myofibril, sarcomere
PTM
Autophosphorylated on serine residues.
More Infomation

Górska, A., & Mazur, A. J. (2022). Integrin-linked kinase (ILK): the known vs. the unknown and perspectives. Cellular and Molecular Life Sciences, 79(2), 100.

McDonald, P. C., & Dedhar, S. (2022). New perspectives on the role of integrin-linked kinase (ILK) signaling in cancer metastasis. Cancers, 14(13), 3209.

Martucci, N., Michalopoulos, G. K., & Mars, W. M. (2021). Integrin linked kinase (ILK) and its role in liver pathobiology. Gene Expression, 20(3), 201.

Yue, Y., Wang, C., Benedict, C., Huang, G., Truongcao, M., Roy, R., ... & Kishore, R. (2020). Interleukin-10 deficiency alters endothelial progenitor cell–derived exosome reparative effect on myocardial repair via integrin-linked kinase enrichment. Circulation research, 126(3), 315-329.

Tsirtsaki, K., & Gkretsi, V. (2020). The focal adhesion protein Integrin-Linked Kinase (ILK) as an important player in breast cancer pathogenesis. Cell Adhesion & Migration, 14(1), 204-213.

Zheng, C. C., Hu, H. F., Hong, P., Zhang, Q. H., Xu, W. W., He, Q. Y., & Li, B. (2019). Significance of integrin-linked kinase (ILK) in tumorigenesis and its potential implication as a biomarker and therapeutic target for human cancer. American journal of cancer research, 9(1), 186.

Brodehl, A., Rezazadeh, S., Williams, T., Munsie, N. M., Liedtke, D., Oh, T., ... & FORGE Canada Consortium. (2019). Mutations in ILK, encoding integrin-linked kinase, are associated with arrhythmogenic cardiomyopathy. Translational Research, 208, 15-29.

Gulzar, M., Syed, S. B., Khan, F. I., Khan, P., Ali, S., Hasan, G. M., ... & Hassan, M. I. (2019). Elucidation of interaction mechanism of ellagic acid to the integrin linked kinase. International journal of biological macromolecules, 122, 1297-1304.

Karachaliou, N., Cardona, A. F., Bracht, J. W. P., Aldeguer, E., Drozdowskyj, A., Fernandez-Bruno, M., ... & Rosell, R. (2019). Integrin-linked kinase (ILK) and src homology 2 domain-containing phosphatase 2 (SHP2): Novel targets in EGFR-mutation positive non-small cell lung cancer (NSCLC). EBioMedicine, 39, 207-214.

de Frutos, S., Luengo, A., García-Jérez, A., Hatem-Vaquero, M., Griera, M., O'Valle, F., ... & Calleros, L. (2019). Chronic kidney disease induced by an adenine rich diet upregulates integrin linked kinase (ILK) and its depletion prevents the disease progression. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(6), 1284-1297.

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For research use only. Not intended for any clinical use.

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