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Mouse Anti-HORMAD1 (AA 1-395) Recombinant Antibody (CBFYH-1718) (CBMAB-H2721-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1718
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-395

Target

Full Name
HORMA domain containing 1
Introduction
This gene encodes a HORMA domain-containing protein. HORMA domains are involved in chromatin binding and play a role in cell cycle regulation. The encoded protein may play a role in meiosis, and expression of this gene is a potential marker for cancer. A pseudogene of this gene is located on the long arm of chromosome 6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Entrez Gene ID
84072
UniProt ID
Q86X24
Alternative Names
HORMA Domain Containing 1; Cancer/Testis Antigen 46; Newborn Ovary HORMA Protein; NOHMA; CT46; Testis Tissue Sperm-Binding Protein Li 86P; HORMA Domain-Containing Protein 1
Function
Plays a key role in meiotic progression. Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends. Promotes synaptonemal-complex formation independently of its role in homology search. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes.
Biological Process
Blastocyst development Source: UniProtKB
Meiotic cell cycle Source: UniProtKB
Meiotic DNA double-strand break formation Source: UniProtKB
Meiotic recombination checkpoint signaling Source: UniProtKB
Meiotic sister chromatid cohesion Source: UniProtKB
Oogenesis Source: UniProtKB
Regulation of homologous chromosome segregation Source: UniProtKB
Spermatogenesis Source: UniProtKB
Synaptonemal complex assembly Source: UniProtKB
Cellular Location
Nucleus; Chromosome. Preferentially localizes to unsynapsed or desynapsed chromosomal regions during the prophase I stage of meiosis. TRIP13 is required for depletion from synapsed chromosomes. The expression of the phosphorylated form at Ser-377 is restricted to unsynapsed chromosomal regions (By similarity).
PTM
Phosphorylated at Ser-377 in a SPO11-dependent manner.
More Infomation

Okutman, O., Boivin, M., Muller, J., Charlet-Berguerand, N., & Viville, S. (2023). A biallelic loss of function variant in HORMAD1 within a large consanguineous Turkish family is associated with spermatogenic arrest. Human Reproduction, 38(2), 306-314.

Tarantino, D., Walker, C., Weekes, D., Pemberton, H., Davidson, K., Torga, G., ... & NJ Tutt, A. (2022). Functional screening reveals HORMAD1-driven gene dependencies associated with translesion synthesis and replication stress tolerance. Oncogene, 41(32), 3969-3977.

Liu, K., Cheng, L., Zhu, K., Wang, J., & Shu, Q. (2022). The cancer/testis antigen HORMAD1 mediates epithelial–mesenchymal transition to promote tumor growth and metastasis by activating the Wnt/β-catenin signaling pathway in lung cancer. Cell Death Discovery, 8(1), 136.

Zong, B., Sun, L., Peng, Y., Wang, Y., Yu, Y., Lei, J., ... & Liu, S. (2021). HORMAD1 promotes docetaxel resistance in triple negative breast cancer by enhancing DNA damage tolerance Corrigendum in/10.3892/or. 2021.8146. Oncology reports, 46(1), 1-15.

Liu, K., Wang, Y., Zhu, Q., Li, P., Chen, J., Tang, Z., ... & Liu, Y. (2020). Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8–MCM9 complex and compromises DNA mismatch repair in cancer cells. Cell death & disease, 11(7), 519.

Fujiwara, Y., Horisawa-Takada, Y., Inoue, E., Tani, N., Shibuya, H., Fujimura, S., ... & Ishiguro, K. I. (2020). Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. PLoS genetics, 16(9), e1009048.

Carofiglio, F., Sleddens-Linkels, E., Wassenaar, E., Inagaki, A., van Cappellen, W. A., Grootegoed, J. A., ... & Baarends, W. M. (2018). Repair of exogenous DNA double-strand breaks promotes chromosome synapsis in SPO11-mutant mouse meiocytes, and is altered in the absence of HORMAD1. DNA repair, 63, 25-38.

Nichols, B. A., Oswald, N. W., McMillan, E. A., McGlynn, K., Yan, J., Kim, M. S., ... & Whitehurst, A. W. (2018). HORMAD1 is a negative prognostic indicator in lung adenocarcinoma and specifies resistance to oxidative and genotoxic stress. Cancer research, 78(21), 6196-6208.

Gao, Y., Kardos, J., Yang, Y., Tamir, T. Y., Mutter-Rottmayer, E., Weissman, B., ... & Vaziri, C. (2018). The cancer/testes (CT) antigen HORMAD1 promotes homologous recombinational DNA repair and radioresistance in lung adenocarcinoma cells. Scientific reports, 8(1), 15304.

Wang, X., Tan, Y., Cao, X., Kim, J. A., Chen, T., Hu, Y., ... & Wang, X. (2018). Epigenetic activation of HORMAD1 in basal-like breast cancer: role in Rucaparib sensitivity. Oncotarget, 9(53), 30115.

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For research use only. Not intended for any clinical use.

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