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Rabbit Anti-HNRNPF Recombinant Antibody (EG1531) (CBMAB-EN1830-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1531
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from N-terminal of human hnRNP F.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
heterogeneous nuclear ribonucleoprotein F
Introduction
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Entrez Gene ID
Human3185
Mouse98758
Rat64200
UniProt ID
HumanP52597
MouseQ9Z2X1
RatQ794E4
Alternative Names
HNRPF; mcs94-1; OK/SW-cl.23
Function
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Biological Process
mRNA splicing, via spliceosome Source: UniProtKB
Regulation of RNA splicing Source: UniProtKB
RNA processing Source: ProtInc
Cellular Location
Nucleoplasm
PTM
Sumoylated.
More Infomation

Huang, H., Li, Y., Zhang, G., Ruan, G. X., Zhu, Z., Chen, W., ... & Ou, X. (2023). The RNA-binding protein hnRNP F is required for the germinal center B cell response. Nature Communications, 14(1), 1731.

Brownmiller, T., & Caplen, N. J. (2023). The HNRNPF/H RNA binding proteins and disease. Wiley Interdisciplinary Reviews: RNA, e1788.

Zhang, A., Sun, Y., Jing, H., Liu, J., Duan, E., Ke, W., ... & Zhang, Y. (2022). Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication. Virology Journal, 19(1), 82.

Montero‐Conde, C., Graña‐Castro, O., Martín‐Serrano, G., Martínez‐Montes, Á. M., Zarzuela, E., Muñoz, J., ... & Robledo, M. (2020). Hsa‐miR‐139‐5p is a prognostic thyroid cancer marker involved in HNRNPF‐mediated alternative splicing. International journal of cancer, 146(2), 521-530.

Xu, C., Xie, N., Su, Y., Sun, Z., Liang, Y., Zhang, N., ... & Chen, J. (2020). HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death & Differentiation, 27(6), 1998-2013.

Li, F., Zhao, H., Su, M., Xie, W., Fang, Y., Du, Y., ... & Tan, W. (2019). HnRNP-F regulates EMT in bladder cancer by mediating the stabilization of Snail1 mRNA by binding to its 3′ UTR. EBioMedicine, 45, 208-219.

Li, F., Su, M., Zhao, H., Xie, W., Cao, S., Xu, Y., ... & Tan, W. (2019). HnRNP-F promotes cell proliferation by regulating TPX2 in bladder cancer. American Journal of Translational Research, 11(11), 7035.

Tyson-Capper, A., & Gautrey, H. (2018). Regulation of Mcl-1 alternative splicing by hnRNP F, H1 and K in breast cancer cells. RNA biology, 15(12), 1448-1457.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell Death & Disease, 9(5), 479.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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