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Mouse Anti-HIPK1 (AA 439-697) Recombinant Antibody (CBFYH-1126) (CBMAB-H2079-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1126
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 439-697 of human HIPK1 produced in E.coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 439-697

Target

Full Name
homeodomain interacting protein kinase 1
Introduction
The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms.
Entrez Gene ID
204851
UniProt ID
Q86Z02
Alternative Names
Homeodomain Interacting Protein Kinase 1; Nuclear Body-Associated Kinase 2; EC 2.7.11.1; Nbak2; Myak; Homeodomain Interacting Protein Kinase 1-Like Protein; Homeodomain-Interacting Protein Kinase 1; Nuclear Body Associated Kinase 2b; KIAA0630
Function
Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed:24559171).
Biological Process
Adherens junction assembly Source: Ensembl
Anterior/posterior pattern specification Source: Ensembl
Definitive hemopoiesis Source: UniProtKB
Embryonic camera-type eye morphogenesis Source: Ensembl
Embryonic retina morphogenesis in camera-type eye Source: Ensembl
Endothelial cell apoptotic process Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: UniProtKB
Eye development Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: GO_Central
Iris morphogenesis Source: Ensembl
Lens induction in camera-type eye Source: Ensembl
Neuron differentiation Source: Ensembl
Peptidyl-serine phosphorylation Source: GO_Central
Peptidyl-threonine phosphorylation Source: GO_Central
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Protein phosphorylation Source: UniProtKB
Regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Retina layer formation Source: Ensembl
Smoothened signaling pathway Source: GO_Central
Cellular Location
Nucleus; Nucleus speckle; Cytoplasm. Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation.
PTM
Autophosphorylated. Phosphorylated and activated by JNK1.
Degraded by PARK7 at the protein level.
Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm.
More Infomation

Bei, Y., Zhu, Y., Wei, M., Yin, M., Li, L., Chen, C., ... & Xiao, J. (2023). HIPK1 Inhibition Protects against Pathological Cardiac Hypertrophy by Inhibiting the CREB‐C/EBPβ Axis. Advanced Science, 2300585.

Gong, J., Zhao, S., Luo, S., Yin, S., Li, X., & Feng, Y. (2022). Downregulation of circ‐ZNF644 alleviates LPS‐induced HK2 cell injury via miR‐335‐5p/HIPK1 axis. Environmental Toxicology, 37(12), 2855-2864.

Wu, Q., Yang, H., Tai, R., Li, C., Xia, T., Liu, Y., & Sun, C. (2022). Lnc‐hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR‐497. BioFactors, 48(1), 135-147.

Zhang, D., Li, Y., & Sun, P. (2020). miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1. Experimental and Therapeutic Medicine, 19(1), 339-346.

Meng, L., Zhao, X., & Zhang, H. (2019). HIPK1 interference attenuates inflammation and oxidative stress of acute lung injury via autophagy. Medical science monitor: international medical journal of experimental and clinical research, 25, 827.

Liu, B., Du, R., Zhou, L., Xu, J., Chen, S., Chen, J., ... & Liu, S. (2018). miR-200c/141 regulates breast cancer stem cell heterogeneity via targeting HIPK1/β-catenin axis. Theranostics, 8(21), 5801.

Conte, A., & Pierantoni, G. M. (2018). Update on the regulation of HIPK1, HIPK2 and HIPK3 protein kinases by microRNAs. Microrna, 7(3), 178-186.

Inwood, S., Buehler, E., Betenbaugh, M., Lal, M., & Shiloach, J. (2018). Identifying HIPK1 as target of miR‐22‐3p enhancing recombinant protein production from HEK 293 cell by using microarray and HTP siRNA screen. Biotechnology journal, 13(2), 1700342.

Zhou, B., Yu, Y., Yu, L., Que, B., & Qiu, R. (2018). Sipi soup inhibits cancer‑associated fibroblast activation and the inflammatory process by downregulating long non‑coding RNA HIPK1‑AS. Molecular Medicine Reports, 18(2), 1361-1368.

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For research use only. Not intended for any clinical use.

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