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Mouse Anti-HIF1AN Recombinant Antibody (FIH162C) (CBMAB-H2048-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
FIH162C
Antibody Isotype
IgG1
Application
IHC-P, WB

Basic Information

Immunogen
Full length Human FIH expressed in E. coli BL21 cells
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor
Introduction
HIF1AN (Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) is a Protein Coding gene. Diseases associated with HIF1AN include Hypoxia and Anemia Of Prematurity. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and HIF-2-alpha transcription factor network. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors. An important paralog of this gene is HSPBAP1.
Entrez Gene ID
55662
UniProt ID
Q9NWT6
Alternative Names
Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor; Hypoxia-Inducible Factor Asparagine Hydroxylase; Peptide-Aspartate Beta-Dioxygenase; Factor Inhibiting HIF-1; FIH-1; FIH1
Function
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Biological Process
Negative regulation of Notch signaling pathway Source: UniProtKB
Negative regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: UniProtKB
Peptidyl-asparagine hydroxylation Source: UniProtKB
Peptidyl-aspartic acid hydroxylation Source: UniProtKB
Peptidyl-histidine hydroxylation Source: UniProtKB
Positive regulation of myoblast differentiation Source: UniProtKB
Positive regulation of vasculogenesis Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Perinuclear region. Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.
More Infomation

Kunej, T. (2021). Integrative map of HIF1A regulatory elements and variations. Genes, 12(10), 1526.

He, R., Wang, Z., Cui, M., Liu, S., Wu, W., Chen, M., ... & Shao, Z. (2021). HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy. Autophagy, 17(11), 3338-3360.

Lin, Q., Li, S., Jiang, N., Jin, H., Shao, X., Zhu, X., ... & Ni, Z. (2021). Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy. Autophagy, 17(10), 2975-2990.

Xu, F., Huang, M., Chen, Q., Niu, Y., Hu, Y., Hu, P., ... & Zhao, G. (2021). LncRNA HIF1A-AS1 promotes gemcitabine resistance of pancreatic cancer by enhancing glycolysis through modulating the AKT/YB1/HIF1α pathway. Cancer Research, 81(22), 5678-5691.

Tiwari, A., Tashiro, K., Dixit, A., Soni, A., Vogel, K., Hall, B., ... & Bagchi, A. (2020). Loss of HIF1A from pancreatic cancer cells increases expression of PPP1R1B and degradation of p53 to promote invasion and metastasis. Gastroenterology, 159(5), 1882-1897.

Ou, Z. L., Luo, Z., Wei, W., Liang, S., Gao, T. L., & Lu, Y. B. (2019). Hypoxia-induced shedding of MICA and HIF1A-mediated immune escape of pancreatic cancer cells from NK cells: role of circ_0000977/miR-153 axis. RNA biology, 16(11), 1592-1603.

Yin, N., Zhu, L., Ding, L., Yuan, J., Du, L., Pan, M., ... & Xiao, H. (2019). MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. Cellular & molecular biology letters, 24, 1-11.

Cimmino, F., Avitabile, M., Lasorsa, V. A., Montella, A., Pezone, L., Cantalupo, S., ... & Capasso, M. (2019). HIF-1 transcription activity: HIF1A driven response in normoxia and in hypoxia. BMC medical genetics, 20, 1-15.

Ebersole, J. L., Novak, M. J., Orraca, L., Martinez‐Gonzalez, J., Kirakodu, S., Chen, K. C., ... & Gonzalez, O. A. (2018). Hypoxia‐inducible transcription factors, HIF1A and HIF2A, increase in aging mucosal tissues. Immunology, 154(3), 452-464.

Wang, R., Ma, Z., Feng, L., Yang, Y., Tan, C., Shi, Q., ... & Fang, J. (2018). LncRNA MIR31HG targets HIF1A and P21 to facilitate head and neck cancer cell proliferation and tumorigenesis by promoting cell-cycle progression. Molecular cancer, 17(1), 1-6.

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For research use only. Not intended for any clinical use.

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