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Mouse Anti-GSKIP Recombinant Antibody (CBFYH-0509) (CBMAB-H1382-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0509
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
GSK3B Interacting Protein
Introduction
This gene encodes a protein that is involved as a negative regulator of GSK3-beta in the Wnt signaling pathway. The encoded protein may play a role in the retinoic acid signaling pathway by regulating the functional interactions between GSK3-beta, beta-catenin and cyclin D1, and it regulates the beta-catenin/N-cadherin pool. The encoded protein contains a GSK3-beta interacting domain (GID) in its C-terminus, which is similar to the GID of Axin. The protein also contains an evolutionarily conserved RII-binding domain, which facilitates binding with protein kinase-A and GSK3-beta, enabling its role as an A-kinase anchoring protein. Alternatively spliced transcript variants have been observed for this gene.
Entrez Gene ID
51527
UniProt ID
Q9P0R6
Alternative Names
GSK3B Interacting Protein; GSK3beta Interaction Protein; C14orf129; Chromosome 14 Open Reading Frame 129; GSK3-Beta Interaction Protein; GSK3B-Interacting Protein; HSPC210
Function
A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets (PubMed:27484798, PubMed:25920809, PubMed:16981698).

The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively (PubMed:27484798, PubMed:16981698).

Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B (PubMed:25920809).

During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (PubMed:19830702).
Biological Process
Intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Regulation of Wnt signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus
PTM
Phosphorylated by GSK3B.
More Infomation

Tsai, C. Y., Ko, H. J., Chiou, S. J., Lin, X. Y., Chuang, T. H., Cheng, J. T., ... & Hong, Y. R. (2023). GSKIP modulates cell aggregation through EMT/MET signaling rather than differentiation in SH-SY5Y human neuroblastoma cells. Journal of Cell Communication and Signaling, 1-16.

Wu, N. S., Lin, Y. F., Ma, I. C., Ko, H. J., & Hong, Y. R. (2022). Many faces and functions of GSKIP: a temporospatial regulation view. Cellular Signalling, 110391.

Pegliasco, J., Hirsch, P., Marzac, C., Isnard, F., Meniane, J. C., Deswarte, C., ... & Bellanne-Chantelot, C. (2022). Germline ATG2B/GSKIP-containing 14q32 duplication predisposes to early clonal hematopoiesis leading to myeloid neoplasms. Leukemia, 36(1), 126-137.

Sakai, S. S., Hasegawa, A., Ishimura, R., Tamura, N., Kageyama, S., Komatsu-Hirota, S., ... & Komatsu, M. (2022). Loss of Atg2b and Gskip impairs the maintenance of the hematopoietic stem cell pool size. Molecular and cellular biology, 42(1), e00024-21.

Tsai, C. Y., Chiou, S. J., Ko, H. J., Cheng, Y. F., Lin, S. Y., Lai, Y. L., ... & Hong, Y. R. (2022). Deciphering the evolution of composite-type GSKIP in mitochondria and Wnt signaling pathways. Plos one, 17(1), e0262138.

Kong, S., Xue, H., Li, Y., Li, P., Ma, F., Liu, M., & Li, W. (2020). The long noncoding RNA OTUD6B-AS1 enhances cell proliferation and the invasion of hepatocellular carcinoma cells through modulating GSKIP/Wnt/β-catenin signalling via the sequestration of miR-664b-3p. Experimental Cell Research, 395(1), 112180.

Li, N., Cheng, C., & Wang, T. (2020). MiR-181c-5p mitigates tumorigenesis in cervical squamous cell carcinoma via targeting glycogen synthase kinase 3β interaction protein (GSKIP). OncoTargets and therapy, 13, 4495.

Ko, H. J., Chiou, S. J., Wong, Y. H., Wang, Y. H., Lai, Y. L., Chou, C. H., ... & Hong, Y. R. (2019). GSKIP-mediated anchoring increases phosphorylation of tau by PKA but not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau axis signaling in cerebrospinal fluid and iPS cells in Alzheimer disease. Journal of clinical medicine, 8(10), 1751.

Chou, C. H., Yang, M. C., Hsiao, B. X., Wang, Y. H., Liu, H. F., Chiou, S. J., ... & Hong, Y. R. (2018). The origin of GSKIP, a multifaceted regulatory factor in the mammalian Wnt pathway. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1865(8), 1046-1059.

Babushok, D. V., Stanley, N. L., Morrissette, J. J., Lieberman, D. B., Olson, T. S., Chou, S. T., & Hexner, E. O. (2018). Germline duplication of ATG2B and GSKIP genes is not required for the familial myeloid malignancy syndrome associated with the duplication of chromosome 14q32. Leukemia, 32(12), 2720-2723.

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For research use only. Not intended for any clinical use.

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