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Mouse Anti-GRB2 Recombinant Antibody (CBLG1-1705) (CBMAB-G4812-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLG1-1705
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
growth factor receptor bound protein 2
Introduction
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
2885
UniProt ID
P62993
Alternative Names
Growth Factor Receptor Bound Protein 2; Growth Factor Receptor-Bound Protein 2; SH2/SH3 Adapter GRB2; Protein Ash; ASH; Epidermal Growth Factor Receptor-Binding Protein GRB2; Growth Factor Receptor-Bound Protein 3; Abundant SRC Homology;
Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

Isoform 2:
Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Biological Process
Actin cytoskeleton reorganization Source: UniProtKB
Aging Source: Ensembl
Anatomical structure formation involved in morphogenesis Source: Ensembl
Branching involved in labyrinthine layer morphogenesis Source: Ensembl
Cell differentiation Source: Ensembl
Cellular response to ionizing radiation Source: BHF-UCL
Epidermal growth factor receptor signaling pathway Source: UniProtKB
Insulin receptor signaling pathway Source: UniProtKB
Positive regulation of actin filament polymerization Source: Ensembl
Positive regulation of reactive oxygen species metabolic process Source: BHF-UCL
Ras protein signal transduction Source: UniProtKB
Receptor internalization Source: BHF-UCL
Regulation of MAPK cascade Source: Ensembl
Signal transduction in response to DNA damage Source: BHF-UCL
Cellular Location
Endosome; Golgi apparatus; Cytoplasm; Nucleus
More Infomation

Chang, S. Y., Han, S. Z., Choe, H. M., Gao, K., Jin, Z. Y., Liu, X. Y., ... & Kang, J. D. (2022). miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. The International Journal of Biochemistry & Cell Biology, 147, 106212.

Yu, Z., Xu, Y., Du, L., Zhang, F., Shao, M., Xie, L., ... & Lyu, F. (2022). Synergistic effect of growth factor receptor-bound protein 2/epidermal growth factor receptor dual-targeting peptide inhibitor and salinomycin on osteosarcoma. Journal of Cancer Research and Therapeutics, 18(2), 352-361.

Liao, Q., & Gao, X. (2022). Tribbles homolog 3 contributes to high glucose-induced injury in retinal pigment epithelial cells via binding to growth factor receptor-bound 2. Bioengineered, 13(4), 10386-10398.

Lara, O. D., Rodriguez-Aguayo, C., Bayraktar, E., Amero, P., Ma, S., Mangala, L. S., ... & Sood, A. K. (2020). A needle in a haystack: Targeting growth factor receptor bound protein-2 (Grb2) as a novel therapeutic in uterine carcinoma. Gynecologic Oncology, 159, 229-230.

Lewis, T. R., Smith, J., Griffin, K., Aguiar, S., Rueb, K. F., Holmberg-Douglas, N., ... & Arpin, C. C. (2020). NHD2-15, a novel antagonist of Growth Factor Receptor-Bound Protein-2 (GRB2), inhibits leukemic proliferation. Plos one, 15(8), e0236839.

Zhao, X. R., Zhang, Z., Gao, M., Li, L., Sun, P. Y., Xu, L. N., ... & Peng, J. Y. (2020). MicroRNA-27a-3p aggravates renal ischemia/reperfusion injury by promoting oxidative stress via targeting growth factor receptor-bound protein 2. Pharmacological Research, 155, 104718.

Bolgov, A., Korban, S., Luzik, D., Zhemkov, V., Kim, M., Rogacheva, O., & Bezprozvanny, I. (2020). Crystal structure of the SH3 domain of growth factor receptor-bound protein 2. Acta Crystallographica Section F: Structural Biology Communications, 76(6), 263-270.

Li, C., Bademci, G., Subasioglu, A., Diaz-Horta, O., Zhu, Y., Liu, J., ... & Tekin, M. (2019). Dysfunction of GRAP, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss. Proceedings of the National Academy of Sciences, 116(4), 1347-1352.

Fares, M., Mohamed, H. T., Ibrahim, S. A., Hosney, M., Rady, M. I., El-Shinawi, M., & Mohamed, M. M. (2019). Incidence of human cytomegalovirus in breast carcinoma tissues is associated with a higher expression of growth factor receptor-bound protein 2. The Egyptian Journal of Hospital Medicine, 75(3), 2358-2365.

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For research use only. Not intended for any clinical use.

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