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Rabbit Anti-GZMA Recombinant Antibody (EG1425) (CBMAB-EN1704-LY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG1425
Antibody Isotype
IgG
Application
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GRAA.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
granzyme A
Introduction
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.
Entrez Gene ID
3001
UniProt ID
P12544
Alternative Names
HFSP; CTLA3
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3257574, PubMed:3262682, PubMed:3263427, PubMed:32299851, PubMed:12819770).

It cleaves after Lys or Arg (PubMed:32299851, PubMed:12819770).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed:32299851).

Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed:12524539).

Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed:11555662, PubMed:12628186, PubMed:16818237).
Biological Process
Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Cytotoxic T cell pyroptotic process Source: UniProtKB
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Immune response Source: UniProtKB
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of endodeoxyribonuclease activity Source: UniProtKB
Negative regulation of oxidoreductase activity Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB
Response to bacterium Source: Ensembl
Cellular Location
Isoform alpha: Secreted; Cytoplasmic granule. Delivered into the target cell by perforin.
More Infomation

Cheng, Z., Thompson, E. J., Mendive‐Tapia, L., Scott, J. I., Benson, S., Kitamura, T., ... & Vendrell, M. (2023). Fluorogenic Granzyme A Substrates Enable Real‐Time Imaging of Adaptive Immune Cell Activity. Angewandte Chemie, 135(8), e202216142.

Moreno-Martinez, L., Santiago, L., de la Torre, M., Calvo, A. C., Pardo, J., & Osta, R. (2022). Hemizygous Granzyme A Mice Expressing the hSOD1G93A Transgene Show Slightly Extended Lifespan. International Journal of Molecular Sciences, 23(21), 13554.

Rawle, D. J., Le, T. T., Dumenil, T., Bishop, C., Yan, K., Nakayama, E., ... & Suhrbier, A. (2022). Widespread discrepancy in Nnt genotypes and genetic backgrounds complicates granzyme A and other knockout mouse studies. Elife, 11, e70207.

Garzón-Tituaña, M., Sierra-Monzón, J. L., Comas, L., Santiago, L., Khaliulina-Ushakova, T., Uranga-Murillo, I., ... & Arias, M. (2021). Granzyme A inhibition reduces inflammation and increases survival during abdominal sepsis. Theranostics, 11(8), 3781.

Di Censo, C., Marotel, M., Mattiola, I., Müller, L., Scarno, G., Pietropaolo, G., ... & Sciumè, G. (2021). Granzyme A and CD160 expression delineates ILC1 with graded functions in the mouse liver. European Journal of Immunology, 51(11), 2568-2575.

Santiago, L., Castro, M., Sanz-Pamplona, R., Garzon, M., Ramirez-Labrada, A., Tapia, E., ... & Pardo, J. (2020). Extracellular granzyme A promotes colorectal cancer development by enhancing gut inflammation. Cell reports, 32(1).

Zhou, Z., He, H., Wang, K., Shi, X., Wang, Y., Su, Y., ... & Shao, F. (2020). Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells. Science, 368(6494), eaaz7548.

Schanoski, A. S., Le, T. T., Kaiserman, D., Rowe, C., Prow, N. A., Barboza, D. D., ... & Suhrbier, A. (2020). Granzyme A in chikungunya and other arboviral infections. Frontiers in immunology, 10, 3083.

Shimizu, K., Yamasaki, S., Sakurai, M., Yumoto, N., Ikeda, M., Mishima-Tsumagari, C., ... & Fujii, S. I. (2019). Granzyme A stimulates pDCs to promote adaptive immunity via induction of type I IFN. Frontiers in immunology, 10, 1450.

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For research use only. Not intended for any clinical use.

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