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Rabbit Anti-GPR35 Recombinant Antibody (EG1401) (CBMAB-EN1680-LY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG1401
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPR35.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 35
Entrez Gene ID
2859
UniProt ID
Q9HC97
Function
Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.
Biological Process
Chemokine-mediated signaling pathway Source: MGI
Cytoskeleton organization Source: Ensembl
G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of neuronal action potential Source: Ensembl
Negative regulation of voltage-gated calcium channel activity Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling Pathway Source: GO_Central
Positive regulation of Rho protein signal transduction Source: GO_Central
Cellular Location
Cell membrane. Internalized to the cytoplasm after exposure to kynurenic acid.
Topology
Extracellular: 1-24
Helical: 25-45
Cytoplasmic: 46-56
Helical: 57-77
Extracellular: 78-90
Helical: 91-112
Cytoplasmic: 113-135
Helical: 136-156
Extracellular: 157-174
Helical: 175-195
Cytoplasmic: 196-218
Helical: 219-239
Extracellular: 240-258
Helical: 259-279
Cytoplasmic: 280-309
More Infomation

Milligan, G. (2023). GPR35: from enigma to therapeutic target. Trends in Pharmacological Sciences.

Wei, X., Yin, F., Wu, M., Xie, Q., Zhao, X., Zhu, C., ... & Wang, X. (2023). G protein-coupled receptor 35 attenuates nonalcoholic steatohepatitis by reprogramming cholesterol homeostasis in hepatocytes. Acta Pharmaceutica Sinica B, 13(3), 1128-1144.

Wyant, G. A., Yu, W., Doulamis, I. P., Nomoto, R. S., Saeed, M. Y., Duignan, T., ... & Kaelin Jr, W. G. (2022). Mitochondrial remodeling and ischemic protection by G protein–coupled receptor 35 agonists. Science, 377(6606), 621-629.

Chen, Z., Jiao, Y., Zhang, Y., Wang, Q., Wu, W., Zheng, J., & Li, J. (2022). G-protein coupled receptor 35 induces intervertebral disc degeneration by mediating the influx of calcium ions and upregulating reactive oxygen species. Oxidative Medicine and Cellular Longevity, 2022.

Nesci, S. (2022). GPR35, ally of the anti-ischemic ATPIF1-ATP synthase interaction. Trends in Pharmacological Sciences.

Li, H., Nguyen, H., Meda Venkata, S. P., Koh, J. Y., Kowluru, A., Li, L., ... & Wang, J. M. (2021). Novel Role of GPR35 (G-Protein–Coupled Receptor 35) in the Regulation of Endothelial Cell Function and Blood Pressure. Hypertension, 78(3), 816-830.

Boleij, A., Fathi, P., Dalton, W., Park, B., Wu, X., Huso, D., ... & Sears, C. L. (2021). G-protein coupled receptor 35 (GPR35) regulates the colonic epithelial cell response to enterotoxigenic Bacteroides fragilis. Communications biology, 4(1), 585.

Quon, T., Lin, L. C., Ganguly, A., Tobin, A. B., & Milligan, G. (2020). Therapeutic opportunities and challenges in targeting the orphan G protein-coupled receptor GPR35. ACS Pharmacology & Translational Science, 3(5), 801-812.

Matysik-Woźniak, A., Wnorowski, A., Turski, W. A., Jóźwiak, K., Jünemann, A., & Rejdak, R. (2019). The presence and distribution of G protein-coupled receptor 35 (GPR35) in the human cornea–evidences from in silico gene expression analysis and immunodetection. Experimental Eye Research, 179, 188-192.

Ali, H., AbdelMageed, M., Olsson, L., Israelsson, A., Lindmark, G., Hammarström, M. L., ... & Sitohy, B. (2019). Utility of G protein-coupled receptor 35 expression for predicting outcome in colon cancer. Tumor Biology, 41(6), 1010428319858885.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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