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Mouse Anti-GPI Recombinant Antibody (CBYC-A968) (CBMAB-A4437-YC)


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Summary

Host Animal
Mouse
Specificity
Rat
Clone
CBYC-A968
Antibody Isotype
IgG
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen
Glucose 6 Phosphate Isomerase
Specificity
Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Glucose-6-Phosphate Isomerase
Introduction
GPI is a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glyc
Entrez Gene ID
292804
UniProt ID
Q6P6V0
Alternative Names
Amf; Gpi; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; Gpi1s; MF; NK; NK/GPI; Nlk; Org; Pgi; Phi
Function
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808).

Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381).

Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:3352745, PubMed:11004567).

It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:3352745, PubMed:11004567).
Biological Process
Carbohydrate metabolic process Source: ProtInc
Erythrocyte homeostasis Source: Ensembl
Gluconeogenesis Source: GO_Central
Glucose 6-phosphate metabolic process Source: UniProtKB
Glucose homeostasis Source: Ensembl
Glycolytic process Source: GO_Central
Hemostasis Source: ProtInc
Humoral immune response Source: ProtInc
In utero embryonic development Source: Ensembl
Learning or memory Source: Ensembl
Mesoderm formation Source: Ensembl
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of immunoglobulin production Source: CAFA
Response to cadmium ion Source: Ensembl
Response to estradiol Source: Ensembl
Response to immobilization stress Source: Ensembl
Response to morphine Source: Ensembl
Response to muscle stretch Source: Ensembl
Response to progesterone Source: Ensembl
Response to testosterone Source: Ensembl
Cellular Location
Secreted; Cytoplasm
Involvement in disease
Hemolytic anemia, non-spherocytic, due to glucose phosphate isomerase deficiency (HA-GPID):
A form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency.
PTM
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
ISGylated.
More Infomation

Li, T., Ge, C., Krämer, A., Sareila, O., Agelii, M. L., Johansson, L., ... & Holmdahl, R. (2023). Pathogenic antibody response to glucose-6-phosphate isomerase targets a modified epitope uniquely exposed on joint cartilage. Annals of the Rheumatic Diseases, 82(6), 799-808.

Guo, Y., Wu, J., Wang, M., Wang, X., Jian, Y., Yang, C., & Guo, W. (2022). The metabolite saccharopine impairs neuronal development by inhibiting the neurotrophic function of glucose-6-phosphate isomerase. Journal of Neuroscience, 42(13), 2631-2646.

Zu, Y., Wang, H., Lin, W., & Zou, C. (2022). Hereditary nonspherocytic hemolytic anemia caused by glucose-6-phosphate isomerase (GPI) deficiency in a Chinese patient: a case report. BMC pediatrics, 22(1), 1-6.

Velmurugan, R., & Incharoensakdi, A. (2021). Overexpression of glucose-6-phosphate isomerase in Synechocystis sp. PCC 6803 with disrupted glycogen synthesis pathway improves exopolysaccharides synthesis. Algal Research, 57, 102357.

Matsumoto, I., Kurata, I., Ohyama, A., Kawaguchi, H., Ebe, H., Osada, A., ... & Sumida, T. (2020). Revisit of autoimmunity to glucose-6-phosphate isomerase in experimental and rheumatoid arthritis. Modern Rheumatology, 30(2), 232-238.

Xu, J., Zhang, X. Y., Li, R., Liu, J., Ye, H., Zhang, X. W., & Li, Z. G. (2020). Glucose-6-phosphate isomerase is associated with disease activity and declines in response to infliximab treatment in rheumatoid arthritis. Chinese Medical Journal, 133(08), 886-891.

Guo, Y., Jiang, W., Yu, W., Niu, X., Liu, F., Zhou, T., ... & Chen, D. (2019). Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility. Journal of proteomics, 208, 103478.

Fermo, E., Vercellati, C., Marcello, A. P., Zaninoni, A., Aytac, S., Cetin, M., ... & Bianchi, P. (2019). Clinical and molecular spectrum of glucose-6-phosphate isomerase deficiency. Report of 12 new cases. Frontiers in Physiology, 10, 467.

Ma, Y. T., Xing, X. F., Dong, B., Cheng, X. J., Guo, T., Du, H., ... & Ji, J. F. (2018). Higher autocrine motility factor/glucose-6-phosphate isomerase expression is associated with tumorigenesis and poorer prognosis in gastric cancer. Cancer management and research, 10, 4969.

Mojzikova, R., Koralkova, P., Holub, D., Saxova, Z., Pospisilova, D., Prochazkova, D., ... & Divoky, V. (2018). Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin. Blood Cells, Molecules, and Diseases, 69, 23-29.

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For research use only. Not intended for any clinical use.

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