Mouse Anti-FBXO43 (AA 1-110) Recombinant Antibody (CBXF-2905) (CBMAB-F3612-CQ)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBXF-2905

Antibody Isotype

IgG

Application

ELISA, WB

Basic Information

Immunogen

FBXO43 (XP_209918, 1aa-110aa) partial recombinant protein with GST tag

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-110

Target

Full Name

F-box protein 43

Introduction

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).

Entrez Gene ID

286151

UniProt ID

Q4G163

Alternative Names

F-Box Protein 43; Endogenous Meiotic Inhibitor 2; EMI2; Early Mitotic Inhibitor 2; F-Box Only Protein 43; FBX43; ERP1;

Research Area

Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable).

Biological Process

Meiotic cell cycle Source: UniProtKB-KW
Negative regulation of meiotic nuclear division Source: GO_Central
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of mitotic nuclear division Source: GO_Central

Cellular Location

Nucleus

PTM

Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation.2 Publications
Ubiquitinated in response to calcium, which promotes proteasomal degradation.

More Infomation

References

Wu, H., Zhang, X., Shen, Q., Liu, Y., Gao, Y., Wang, G., ... & Liu, M. (2022). A homozygous loss‐of‐function mutation in FBXO43 causes human non‐obstructive azoospermia. Clinical Genetics, 101(1), 55-64.

Wang, W., Wang, W., Xu, Y., Shi, J., Fu, J., Chen, B., ... & Sang, Q. (2021). FBXO43 variants in patients with female infertility characterized by early embryonic arrest. Human Reproduction, 36(8), 2392-2402.

Ma, R., Zhu, K., Yuan, D., Gong, M., Li, Y., Li, K., & Meng, L. (2021). Downregulation of the FBXO43 gene inhibits tumor growth in human breast cancer by limiting its interaction with PCNA. Journal of Translational Medicine, 19(1), 1-12.

Ma, Y., Xie, N., Xie, D., Sun, L., Li, S., Li, P., ... & Xie, X. (2019). A novel homozygous FBXO43 mutation associated with male infertility and teratozoospermia in a consanguineous Chinese family. Fertility and sterility, 111(5), 909-917.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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