Mouse Anti-FBXO28 Recombinant Antibody (3E5) (CBMAB-A2885-LY)

Name: Mouse Anti-FBXO28 Recombinant Antibody (3E5)
SKU: CBMAB-A2885-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E5

Antibody Isotype

IgG2a, κ

Application

sELISA, ELISA

Basic Information

Immunogen

FBXO28 (NP_055991, 191 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

F-box protein 28

Introduction

Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM

Entrez Gene ID

23219

UniProt ID

Q9NVF7

Alternative Names

FLJ10766; Fbx28; KIAA0483

Research Area

Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.

Biological Process

Protein polyubiquitination Source: GO_Central

Cellular Location

Kinetochore

More Infomation

References

Phillips, E., Balss, J., Bethke, F., Pusch, S., Christen, S., Hielscher, T., ... & Goidts, V. (2022). PFKFB4 interacts with FBXO28 to promote HIF-1α signaling in glioblastoma. Oncogenesis, 11(1), 1-12.

Schneider, A. L., Myers, C. T., Muir, A. M., Calvert, S., Basinger, A., Perry, M. S., ... & Scheffer, I. E. (2021). FBXO28 causes developmental and epileptic encephalopathy with profound intellectual disability. Epilepsia, 62(1), e13-e21.

Cai, L., Liu, L., Li, L., & Jia, L. (2020). SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation. Cellular Signalling, 65, 109440.

Yu, T., Wang, L., Zhao, C., Qian, B., Yao, C., He, F., ... & Qiu, W. (2019). Sublytic C5b‐9 induces proliferation of glomerular mesangial cells via ERK5/MZF1/RGC‐32 axis activated by FBXO28‐TRAF6 complex. Journal of cellular and molecular medicine, 23(8), 5654-5671.

Yanagishita, T., Yamamoto-Shimojima, K., Nakano, S., Sasaki, T., Shigematsu, H., Imai, K., & Yamamoto, T. (2019). Phenotypic features of 1q41q42 microdeletion including WDR26 and FBXO28 are clinically recognizable: the first case from Japan. Brain and Development, 41(5), 452-455.

Balak, C., Belnap, N., Ramsey, K., Joss, S., Devriendt, K., Naymik, M., ... & Narayanan, V. (2018). A novel FBXO28 frameshift mutation in a child with developmental delay, dysmorphic features, and intractable epilepsy: A second gene that may contribute to the 1q41‐q42 deletion phenotype. American Journal of Medical Genetics Part A, 176(7), 1549-1558.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-FBXO28 (AA 191-290) Recombinant Antibody (CBXF-2901)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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