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Rabbit Anti-EPS15 (AA 448-579) Recombinant Antibody (CBFYE-1078) (CBMAB-E1607-FY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBFYE-1078
Antibody Isotype
IgG
Application
WB, FC, ICC

Basic Information

Immunogen
E.coli-derived recombinant human Eps15, Ala448-Thr579
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 448-579

Target

Full Name
Epidermal Growth Factor Receptor Pathway Substrate 15
Introduction
This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Entrez Gene ID
2060
UniProt ID
P42566
Alternative Names
Epidermal Growth Factor Receptor Pathway Substrate 15; Protein AF-1p; AF1P; Epidermal Growth Factor Receptor Substrate 15; ALL1 Fused Gene From Chromosome 1; Protein Eps15; AF-1P; MLLT5
Research Area
Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Biological Process
Clathrin coat assembly Source: BHF-UCL
Endocytic recycling Source: BHF-UCL
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Golgi to endosome transport Source: UniProtKB
Positive regulation of receptor recycling Source: Ensembl
Postsynaptic neurotransmitter receptor internalization Source: Ensembl
Protein transport Source: UniProtKB-KW
Receptor-mediated endocytosis of virus by host cell Source: CACAO
Regulation of cell population proliferation Source: Ensembl
Vesicle organization Source: UniProtKB
Viral entry into host cell Source: CACAO
Cellular Location
Cytoplasm; Cell membrane; Clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.
Isoform 2: Early endosome membrane. Colocalizes with HGS on bilayered clathrin coats on endosomes.
Involvement in disease
A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with KMT2A/MLL1. The result is a rogue activator protein.
PTM
Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.By similarity
Ubiquitinated.By similarity
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For research use only. Not intended for any clinical use.

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