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Mouse Anti-EPCAM Recombinant Antibody (CBFYE-0950) (CBMAB-E1429-FY)


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Summary

Host Animal
Mouse
Specificity
Rat
Clone
CBFYE-0950
Antibody Isotype
IgG1
Application
FC, IF, WB, IP, IHC

Basic Information

Immunogen
It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas
Specificity
Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Epithelial Cell Adhesion Molecule
Introduction
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Entrez Gene ID
171577
UniProt ID
O55159
Alternative Names
Epithelial Cell Adhesion Molecule; Tumor-Associated Calcium Signal Transducer 1; Major Gastrointestinal Tumor-Associated Protein GA733-2; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Glycoprotein 314; TACSTD1; EGP314; MIC18; TROP1; M4S1; KSA; Membrane Component, Chromosome 4, Surface Marker (35kD Glycoprotein); Antigen Identified By Monoclonal Antibody AUA1; Human Epithelial Glycoprotein-2; Epithelial Cell Surface Antigen;
Research Area
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Biological Process
Cell-cell adhesion via plasma-membrane adhesion molecules Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cell-cell adhesion mediated by cadherin Source: UniProtKB
Positive regulation of cell motility Source: Ensembl
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of stem cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Signal transduction involved in regulation of gene expression Source: UniProtKB
Stem cell differentiation Source: UniProtKB
Ureteric bud development Source: Ensembl
Cellular Location
Lateral cell membrane; Tight junction. Colocalizes with CLDN7 at the lateral cell membrane and tight junction.
Involvement in disease
Diarrhea 5, with tufting enteropathy, congenital (DIAR5):
An intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Hereditary non-polyposis colorectal cancer 8 (HNPCC8):
The disease is caused by variants affecting the gene represented in this entry. HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. An autosomal dominant disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected.
Topology
Extracellular: 24-265
Helical: 266-288
Cytoplasmic: 289-314
PTM
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
More Infomation

Gao, Y., Fan, W. H., Song, Z., Lou, H., & Kang, X. (2021). Comparison of circulating tumor cell (CTC) detection rates with epithelial cell adhesion molecule (EpCAM) and cell surface vimentin (CSV) antibodies in different solid tumors: A retrospective study. PeerJ, 9, e10777.

Fu, J., Shang, Y., Qian, Z., Hou, J., Yan, F., Liu, G., ... & Tian, X. (2021). Chimeric Antigen receptor-T (CAR-T) cells targeting Epithelial cell adhesion molecule (EpCAM) can inhibit tumor growth in ovarian cancer mouse model. Journal of Veterinary Medical Science, 83(2), 241-247.

Gires, O., Pan, M., Schinke, H., Canis, M., & Baeuerle, P. A. (2020). Expression and function of epithelial cell adhesion molecule EpCAM: where are we after 40 years?. Cancer and Metastasis Reviews, 39(3), 969-987.

Eslami-S, Z., Cortés-Hernández, L. E., & Alix-Panabières, C. (2020). Epithelial cell adhesion molecule: an anchor to isolate clinically relevant circulating tumor cells. Cells, 9(8), 1836.

Hosono, H., Ohishi, T., Takei, J., Asano, T., Sayama, Y., Kawada, M., ... & Kato, Y. (2020). The anti‑epithelial cell adhesion molecule (EpCAM) monoclonal antibody EpMab‑16 exerts antitumor activity in a mouse model of colorectal adenocarcinoma. Oncology letters, 20(6), 1-1.

Hu, Y., Wu, Q., Gao, J., Zhang, Y., & Wang, Y. (2019). A meta-analysis and The Cancer Genome Atlas data of prostate cancer risk and prognosis using epithelial cell adhesion molecule (EpCAM) expression. BMC urology, 19(1), 1-8.

Kuan, I. I., Lee, C. C., Chen, C. H., Lu, J., Kuo, Y. S., & Wu, H. C. (2019). The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR–LIN28–LET7 signaling. Journal of Biological Chemistry, 294(19), 7769-7786.

Ni, J., Cozzi, P., Beretov, J., Duan, W., Bucci, J., Graham, P., & Li, Y. (2018). Epithelial cell adhesion molecule (EpCAM) is involved in prostate cancer chemotherapy/radiotherapy response in vivo. BMC cancer, 18(1), 1-12.

Boesch, M., Spizzo, G., & Seeber, A. (2018). Concise review: aggressive colorectal cancer: role of epithelial cell adhesion molecule in cancer stem cells and epithelial-to-mesenchymal transition. Stem cells translational medicine, 7(6), 495-501.

Wang, M. H., Sun, R., Zhou, X. M., Zhang, M. Y., Lu, J. B., Yang, Y., ... & Mai, S. J. (2018). Epithelial cell adhesion molecule overexpression regulates epithelial-mesenchymal transition, stemness and metastasis of nasopharyngeal carcinoma cells via the PTEN/AKT/mTOR pathway. Cell death & disease, 9(1), 1-16.

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For research use only. Not intended for any clinical use.

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