Mouse Anti-EEF1B2 (AA 1-225) Recombinant Antibody (CBFYE-0459) (CBMAB-E0796-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYE-0459

Application

ELISA, WB

Basic Information

Immunogen

EEF1B2 full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human, Mouse, Rat

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-225

Target

Full Name

Eukaryotic Translation Elongation Factor 1 Beta 2

Introduction

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Entrez Gene ID

Human	1933
Mouse	55949
Rat	363241

UniProt ID

Human	P24534
Mouse	O70251
Rat	B5DEN5

Alternative Names

Eukaryotic Translation Elongation Factor 1 Beta 2; EF-1-Beta; EEF1B; EF1B; Eukaryotic Translation Elongation Factor 1 Beta 1; Elongation Factor 1-Beta; EEF1B1

Research Area

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

Biological Process

Response to ethanol Source: Ensembl
Translational elongation Source: GO_Central

Cellular Location

Cytosol; Cytoplasm; Eukaryotic translation elongation factor 1 complex

PTM

Phosphorylation affects the GDP/GTP exchange rate.

More Infomation

References

Gong, P., Liu, J., Jiao, X., Niu, Y., Wang, J., Wang, X., & Yang, Z. (2022). Novel biallelic loss of EEF1B2 function links to autosomal recessive intellectual disability. Human Mutation, 43(3), 299-304.

He, C., Guo, J., Tian, W., & Wong, C. C. (2021). Proteogenomics Integrating Novel Junction Peptide Identification Strategy Discovers Three Novel Protein Isoforms of Human NHSL1 and EEF1B2. Journal of Proteome Research, 20(12), 5294-5303.

Larcher, L., Buratti, J., Héron‐Longe, B., Benzacken, B., Pipiras, E., Keren, B., & Delahaye‐Duriez, A. (2020). New evidence that biallelic loss of function in EEF1B2 gene leads to intellectual disability. Clinical genetics, 97(4), 639-643.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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