Mouse Anti-EEA1 Recombinant Antibody (CF141) (CBMAB-FT341LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CF141

Antibody Isotype

IgG1

Application

ELISA, IF

Basic Information

Immunogen

early endosome antigen 1

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

50% glycerol

Preservative

0.02% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Early Endosome Antigen 1

Entrez Gene ID

8411

UniProt ID

Q15075

Alternative Names

ZFYVE2

Research Area

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Biological Process

Early endosome to late endosome transport Source: UniProtKB
Endocytosis Source: UniProtKB
Synaptic vesicle to endosome fusion Source: UniProtKB
Vesicle fusion Source: UniProtKB
Viral RNA genome replication Source: ParkinsonsUK-UCL

Cellular Location

Early endosome membrane; Cytoplasm

More Infomation

References

McGowan, S. E., & McCoy, D. M. (2021). Neuropilin-1 directs PDGFRα-entry into lung fibroblasts and signaling from very early endosomes. American Journal of Physiology-Lung Cellular and Molecular Physiology, 320(2), L179-L192.

Kamentseva, R., Kosheverova, V., Kharchenko, M., Zlobina, M., Salova, A., Belyaeva, T., ... & Kornilova, E. (2020). Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway. PLoS One, 15(5), e0232532.

Baroja-Mazo, A., Compan, V., Martín-Sánchez, F., Tapia-Abellán, A., Couillin, I., & Pelegrín, P. (2019). Early endosome autoantigen 1 regulates IL-1β release upon caspase-1 activation independently of gasdermin D membrane permeabilization. Scientific reports, 9(1), 1-10.

Posiri, P., Thongsuksangcharoen, S., Chaysri, N., Panyim, S., & Ongvarrasopone, C. (2019). PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon. Fish & shellfish immunology, 95, 449-455.

Miller, C. M., Wan, W. B., Seth, P. P., & Harris, E. N. (2018). Endosomal escape of antisense oligonucleotides internalized by stabilin receptors is regulated by Rab5C and EEA1 during endosomal maturation. Nucleic acid therapeutics, 28(2), 86-96.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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