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Mouse Anti-ECD (AA 1-644) Recombinant Antibody (CBFYE-0399) (CBMAB-E0711-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0399
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
HSGT1 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: MEETMKLATM EDTVEYCLFL IPDESRDSDK HKEILQKYIE RIITRFAPML VPYIWQNQPF NLKYKPGKGG VPAHMFGVTK FGDNIEDEWF IVYVIKQITK EFPELVARIE DNDGEFLLIE AADFLPKWLD PENSTNRVFF CHGELCIIPA PRKSGAESWL PTTPPTIPQA LNIITAHSEK ILASESIRAA VNRRIRGYPE KIQASLHRAH CFLPAGIVAV LKQRPRLVAA AVQAFYLRDP IDLRACRVFK TFLPETRIMT SVTFTKCLYA QLVQQRFVPD RRSGYRLPPP SDPQYRAHEL GMKLAHGFEI LCSKCSPHFS DCKKSLVTAS PLWASFLESL KKNDYFKGLI EGSAQYRERL EMAENYFQLS VDWPESSLAM SPGEEILTLL QTIPFDIEDL KKEAANLPPE DDDQWLDLSP DQLDQLLQEA VGKKESESVS KEEKEQNYDL TEVSESMKAF ISKVSTHKGA ELPREPSEAP ITFDADSFLN YFDKILGPRP NESDSDDLDD EDFECLDSDD DLDFETHEPG EEASLKGTLD NLKSYMAQMD QELAHTCISK SFTTRNQVEP VSQTTDNNSD EEDSGTGESV MAPVDVDLNL VSNILESYSS QAGLAGPASN LLQSMGVQLP DNTDHRPTSK PTKN
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-644

Target

Full Name
ECD
Introduction
Ecdysoneless protein is required for cell autonomous roles in development and oogenesis in Drosophila but the function of its evolutionarily conserved mammalian orthologues is not clear.
Entrez Gene ID
11319
UniProt ID
O95905
Alternative Names
GCR2; SGT1; HSGT1
Research Area
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).

May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).

Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).

The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).

May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Biological Process
Cell population proliferation Source: Ensembl
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
RNA splicing Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Predominantly is located in the cytoplasm.
PTM
Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.
More Infomation

Mirza, S., Kalluchi, A., Raza, M., Saleem, I., Mohapatra, B., Pal, D., ... & Band, V. (2022). Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical Oncogenesis. Molecular Cancer Research, 20(2), 305-318.

Holding, N., Raza, M., Band, V., Mohapatra, B. C., & Band, H. (2022). Novel Interaction Between ECD and EIF4A1 Indicates ECD Regulates Eukaryotic Translation.

Saleem, I., Mirza, S., Sarkar, A., Raza, M., Mohapatra, B., Mushtaq, I., ... & Band, V. (2021). The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export. Molecular and cellular biology, 41(7), e00103-21.

Erkelenz, S., Stanković, D., Mundorf, J., Bresser, T., Claudius, A. K., Boehm, V., ... & Uhlirova, M. (2021). Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic acids research, 49(3), 1688-1707.

Sarkar, A. (2019). Ecdysoneless, a novel regulator of Ca2+ homeostasis and metabolism.

Sarkar, A., Saleem, I., Mirza, S., Bidasee, K., Rao, R., Band, H., & Band, V. (2018). The novel role of ecdysoneless (ECD) in maintain ER Ca2+ homeostasis. Cancer Research, 78(13_Supplement), 448-448.

Saleem, I., Mirza, S., Sarkar, A., Band, H., & Band, V. (2018). Ecdysoneless, a novel regulator of cell cycle progression and oncogenesis. Cancer Research, 78(13_Supplement), 4454-4454.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell death & disease, 9(5), 1-11.

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For research use only. Not intended for any clinical use.

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