Mouse Anti-DMTF1 Antibody (1B7) (CBMAB-0297-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B7

Antibody Isotype

IgG2a

Application

IP, WB, MA

Basic Information

Immunogen

Recombinant peptide

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DMTF1

Introduction

DMTF1 (cyclin D binding myb-like transcription factors 1) is a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. DMTF1 is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. Also, it activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation.

Entrez Gene ID

9988

UniProt ID

Q9Y222

Alternative Names

DMP1; DMTF; hDMP1; MRUL

Function

Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity).

Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity).

Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.

Biological Process

Cell cycle Source: UniProtKB-KW
Regulation of transcription, DNA-templated Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

PTM

Phosphorylated by the cyclin-D2/CDK4, cyclin-D3/CDK4 and cyclin-D2/CDK6 complexes and to a lesser extent by the cyclin-D1/CDK4 complex.

More Infomation

References

Zhai, X., Wu, Y., Zhang, D., Li, H., Chong, T., & Zhao, J. (2021). MiR-6838-5p facilitates the proliferation and invasion of renal cell carcinoma cells through inhibiting the DMTF1/ARF-p53 axis. Journal of Bioenergetics and Biomembranes, 53(2), 191-202.

Li, J., Li, G., Qi, Y., Lu, Y., Wang, H., Shi, K., ... & Sui, G. (2021). SRSF5 regulates alternative splicing of DMTF1 pre-mRNA through modulating SF1 binding. RNA biology, 18(sup1), 318-336.

Fry, E. A., & Inoue, K. (2019). c-MYB and DMTF1 in cancer. Cancer investigation, 37(1), 46-65.

Yang, X., Lou, Y., Wang, M., Liu, C., Liu, Y., & Huang, W. (2019). miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1. Molecular Medicine Reports, 19(3), 1481-1490.

Niklaus, N. J., Humbert, M., & Tschan, M. P. (2018). Cisplatin sensitivity in breast cancer cells is associated with particular DMTF1 splice variant expression. Biochemical and biophysical research communications, 503(4), 2800-2806.

Tian, N., Li, J., Shi, J., & Sui, G. (2017). From general aberrant alternative splicing in cancers and its therapeutic application to the discovery of an oncogenic DMTF1 isoform. International Journal of Molecular Sciences, 18(3), 191.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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