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Mouse Anti-DGAT2 Recombinant Antibody (4C1) (CBMAB-D0762-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4C1
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
DGAT2 (NP_115953.2, 289 a.a. ~ 388 a.a) partial recombinant protein with GST tag. The immunogen sequence: IFEEGSWGRW VQKKFQKYIG FAPCIFHGRG LFSSDTWGLV PYSKPITTVV GEPITIPKLE HPTQQDIDL.htmYMEALVK LFDKHKTKFG LPETEVLEVN
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 289-388

Target

Full Name
diacylglycerol O-acyltransferase homolog 2 (mouse)
Introduction
DGAT2 is one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride.
Entrez Gene ID
84649
UniProt ID
Q96PD7
Alternative Names
Diacylglycerol O-Acyltransferase 2; Acyl-CoA Retinol O-Fatty-Acyltransferase; Diglyceride Acyltransferase 2; EC 2.3.1.20; ARAT; Diacylglycerol O-Acyltransferase Homolog 2 (Mouse); Diacylglycerol O-Acyltransferase-Like Protein 2;
Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed:27184406).

Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity).

Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).

Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
Biological Process
Acylglycerol acyl-chain remodeling Source: Reactome
Bile acid signaling pathway Source: Ensembl
Cellular response to oleic acid Source: BHF-UCL
Cellular triglyceride homeostasis Source: BHF-UCL
Cholesterol homeostasis Source: BHF-UCL
Diacylglycerol biosynthetic process Source: UniProtKB
Diacylglycerol metabolic process Source: BHF-UCL
Fat pad development Source: BHF-UCL
Fatty acid homeostasis Source: BHF-UCL
Glycerol metabolic process Source: UniProtKB-KW
Lipid metabolic process Source: GO_Central
Lipid storage Source: BHF-UCL
Long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
Low-density lipoprotein particle clearance Source: BHF-UCL
Monoacylglycerol biosynthetic process Source: UniProtKB
Negative regulation of fatty acid oxidation Source: Ensembl
Positive regulation of gluconeogenesis Source: Ensembl
Positive regulation of triglyceride biosynthetic process Source: Ensembl
Regulation of cholesterol metabolic process Source: Ensembl
Regulation of lipoprotein metabolic process Source: Ensembl
Regulation of plasma lipoprotein particle levels Source: BHF-UCL
Triglyceride biosynthetic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Lipid droplet; Perinuclear region
Topology
Cytoplasmic: 1-69
Helical: 70-88
Lumenal: 89-92
Helical: 93-112
Cytoplasmic: 113-388
More Infomation

Yang, W., Wang, S., Loor, J. J., Lopes, M. G., Zhao, Y., Ma, X., ... & Xu, C. (2022). Role of diacylglycerol O-acyltransferase (DGAT) isoforms in bovine hepatic fatty acid metabolism. Journal of Dairy Science, 105(4), 3588-3600.

Solé, E., Ros-Freixedes, R., Tor, M., Pena, R. N., & Estany, J. (2021). A sequence variant in the diacylglycerol O-acyltransferase 2 gene influences palmitoleic acid content in pig muscle. Scientific reports, 11(1), 1-10.

Zhong, H., Zhou, Y., Xu, Q., Yan, J., Zhang, X., Zhang, H., ... & Zhou, L. (2021). Low expression of miR-19a-5p is associated with high mRNA expression of diacylglycerol O-acyltransferase 2 (DGAT2) in hybrid tilapia. Genomics, 113(4), 2392-2399.

Solé Mòdol, E., Ros Freixedes, R., Tor i Naudí, M., Pena i Subirà, R. N., & Estany Illa, J. (2021). A sequence variant in the diacylglycerol O-acyltransferase 2 gene influences palmitoleic acid content in pig muscle. Scientific Reports, 2021, vol. 11, núm. 14797.

Loomba, R., Morgan, E., Watts, L., Xia, S., Hannan, L. A., Geary, R. S., ... & Bhanot, S. (2020). Novel antisense inhibition of diacylglycerol O-acyltransferase 2 for treatment of non-alcoholic fatty liver disease: a multicentre, double-blind, randomised, placebo-controlled phase 2 trial. The Lancet Gastroenterology & Hepatology, 5(9), 829-838.

Sciani, J. M., Neves, A., Vassão, R. C., Spencer, P., Antoniazzi, M. M., Jared, C., & Pimenta, D. C. (2019). The amphibian diacylglycerol O-acyltransferase 2 (DGAT2): a ‘paleo-protein’with conserved function but unique folding. The Protein Journal, 38(1), 83-94.

Rosli, R., Chan, P. L., Chan, K. L., Amiruddin, N., Low, E. T. L., Singh, R., ... & Murphy, D. J. (2018). In silico characterization and expression profiling of the diacylglycerol acyltransferase gene family (DGAT1, DGAT2, DGAT3 and WS/DGAT) from oil palm, Elaeis guineensis. Plant Science, 275, 84-96.

Jin, Y., Yuan, Y., Gao, L., Sun, R., Chen, L., Li, D., & Zheng, Y. (2017). Characterization and functional analysis of a type 2 diacylglycerol acyltransferase (DGAT2) gene from oil palm (Elaeis guineensis Jacq.) mesocarp in Saccharomyces cerevisiae and transgenic Arabidopsis thaliana. Frontiers in Plant Science, 8, 1791.

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For research use only. Not intended for any clinical use.

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