Mouse Anti-DCP2 Recombinant Antibody (1D6) (CBMAB-0279-YC)

Name: Mouse Anti-DCP2 Recombinant Antibody (1D6)
SKU: CBMAB-0279-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

1D6

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 273-311 near the C-terminus of human DcpS.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Target

Full Name

DCP2

Entrez Gene ID

167227

UniProt ID

Q8IU60

Function

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12417715, PubMed:12218187, PubMed:12923261, PubMed:21070968, PubMed:28002401).

Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968, PubMed:28002401).

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413).

Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165).

Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968).

Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968).

The presence of a N6-methyladenosine methylation at the second transcribed position of mRNAs (N6,2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (PubMed:28002401).

Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Biological Process

Deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
Histone mRNA catabolic process Source: UniProtKB
mRNA catabolic process Source: UniProtKB
Negative regulation of telomere maintenance via telomerase Source: BHF-UCL
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Regulation of mRNA stability Source: UniProtKB
Regulation of telomerase RNA localization to Cajal body Source: BHF-UCL

Cellular Location

Nucleus; P-body. Predominantly cytoplasmic, in processing bodies (PB) (PubMed:15273322). A minor amount is nuclear (PubMed:15273322).

PTM

Phosphorylated at ser-249 in a MTOR-dependent manner (PubMed:26098573).

More Infomation

References

Kunar, R., & Roy, J. K. (2021). The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila—insights from expression paradigms. Cell and tissue research, 386(2), 261-280.

Wurm, J. P., & Sprangers, R. (2019). Dcp2: an mRNA decapping enzyme that adopts many different shapes and forms. Current Opinion in Structural Biology, 59, 115-123.

Wurm, J. P., Holdermann, I., Overbeck, J. H., Mayer, P. H., & Sprangers, R. (2017). Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme. Proceedings of the National Academy of Sciences, 114(23), 6034-6039.

Charenton, C., Gaudon-Plesse, C., Fourati, Z., Taverniti, V., Back, R., Kolesnikova, O., ... & Graille, M. (2017). A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5′–3′ mRNA exonuclease in yeast. Proceedings of the National Academy of Sciences, 114(45), E9493-E9501.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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