Mouse Anti-DAZAP2 Recombinant Antibody (D0276) (V2LY-0125-LY1129)

Name: Mouse Anti-DAZAP2 Recombinant Antibody (D0276)
SKU: V2LY-0125-LY1129
Rating: 5 (1 reviews)

See all DAZAP2 antibodies

Online Inquiry

Published Data

Mouse Anti-DAZAP2 Recombinant Antibody (D0276) (V2LY-0125-LY1129) in WB

Upregulation of SERPINE1 in DAZAP2/VTA1/KLF5-KO A549-AC cells and downregulation of SERPINE1 in DAZAP2-OE A549-ACE2 cells. mRNA levels of SERPINE1 (upper panel) and protein levels of SERPINE1 (lower panel) were detected by real-time PCR and western blot, respectively. ...View More

Hou, J., Wei, Y., Zou, J., Jaffery, R., Sun, L., Liang, S., ... & Peng, W. (2024). Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection. Nature communications, 15(1), 109. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D0276

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-121 mapping at the N-terminus of human DAZAP2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DAZ associated protein 2

Entrez Gene ID

9802

UniProt ID

Q15038

Cellular Location

Nucleus; Cytoplasm. Predominantly nuclear in macrophages, stimulation of IL17RB with its ligand IL17E induces accumulation in the cytoplasm.

PTM

Ubiquitinated by SMURF2, leading to proteasomal degradation.

More Infomation

References

Liebl, M. C., Moehlenbrink, J., Becker, H., Raddatz, G., Abdeen, S. K., Aqeilan, R. I., ... & Hofmann, T. G. (2021). DAZAP2 acts as specifier of the p53 response to DNA damage. Nucleic acids research, 49(5), 2759-2776.

Yang, L., Li, X., Qin, X., Wang, Q., Zhou, K., Li, H., ... & Li, W. (2019). Deleted in azoospermia-associated protein 2 regulates innate immunity by stimulating Hippo signaling in crab. Journal of Biological Chemistry, 294(40), 14704-14716.

Li, J., Hu, W. X., Luo, S. Q., Xiong, D. H., Sun, S., Wang, Y. P., ... & Hu, J. (2019). Promoter methylation induced epigenetic silencing of DAZAP2, a downstream effector of p38/MAPK pathway, in multiple myeloma cells. Cellular Signalling, 60, 136-145.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DAZAP2 Recombinant Antibody (D0276)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DAZAP2 Recombinant Antibody (3G21)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: