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Mouse Anti-CYGB Recombinant Antibody (77) (V2LY-0125-LY881)

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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
77
Antibody Isotype
IgG2a
Application
WB, IHC, IF, ELISA

Basic Information

Immunogen
Cytoglobin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:20-1:200
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Glycerol & PBS
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytoglobin
Entrez Gene ID
Human114757
Mouse114886
Rat170520
UniProt ID
HumanQ8WWM9
MouseQ9CX80
RatQ921A4
Function
May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.
Biological Process
Oxygen transport Source: Reactome
Regulation of nitric-oxide synthase activity Source: Reactome
Response to oxidative stress Source: UniProtKB
Cellular Location
Cytoplasm
More Infomation

Zweier, J. L., Hemann, C., Kundu, T., Ewees, M. G., Khaleel, S. A., Samouilov, A., ... & El-Mahdy, M. A. (2021). Cytoglobin has potent superoxide dismutase function. Proceedings of the National Academy of Sciences, 118(52).

Dat, N. Q., Thuy, L. T. T., Hieu, V. N., Hai, H., Hoang, D. V., Thi Thanh Hai, N., ... & Kawada, N. (2021). Hexa Histidine–Tagged Recombinant Human Cytoglobin Deactivates Hepatic Stellate Cells and Inhibits Liver Fibrosis by Scavenging Reactive Oxygen Species. Hepatology, 73(6), 2527-2545.

De Backer, J., Maric, D., Bosman, M., Dewilde, S., & Hoogewijs, D. (2021). A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma. Scientific reports, 11(1), 1-11.

Zweier, J. L., & Ilangovan, G. (2020). Regulation of nitric oxide metabolism and vascular tone by cytoglobin. Antioxidants & Redox Signaling, 32(16), 1172-1187.

Le Thi Thanh Thuy, H. H., & Kawada, N. (2020). Role of cytoglobin, a novel radical scavenger, in stellate cell activation and hepatic fibrosis. Clinical and molecular hepatology, 26(3), 280.

Okina, Y., Sato-Matsubara, M., Matsubara, T., Daikoku, A., Longato, L., Rombouts, K., ... & Kawada, N. (2020). TGF-β1-driven reduction of cytoglobin leads to oxidative DNA damage in stellate cells during non-alcoholic steatohepatitis. Journal of hepatology, 73(4), 882-895.

De Backer, J., Razzokov, J., Hammerschmid, D., Mensch, C., Hafideddine, Z., Kumar, N., ... & Dewilde, S. (2018). The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor. Redox biology, 19, 1-10.

Jourd’heuil, F. L., Xu, H., Reilly, T., McKellar, K., El Alaoui, C., Steppich, J., ... & Jourd’heuil, D. (2017). The hemoglobin homolog cytoglobin in smooth muscle inhibits apoptosis and regulates vascular remodeling. Arteriosclerosis, thrombosis, and vascular biology, 37(10), 1944-1955.

Xu, Z. J., Shu, S., Li, Z. J., Liu, Y. M., Zhang, R. Y., & Zhang, Y. (2017). Liuwei Dihuang pill treats diabetic nephropathy in rats by inhibiting of TGF-β/SMADS, MAPK, and NF-kB and upregulating expression of cytoglobin in renal tissues. Medicine, 96(3).

Liu, X., El-Mahdy, M. A., Boslett, J., Varadharaj, S., Hemann, C., Abdelghany, T. M., ... & Zweier, J. L. (2017). Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall. Nature communications, 8(1), 1-14.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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