Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CYBB Recombinant Antibody (NL7) (V2LY-0125-LY853)


See all CYBB antibodies
Online Inquiry
Published Data
V2LY-0125-LY853-1
Mouse Anti-CYBB Recombinant Antibody (NL7) (V2LY-0125-LY853) in Immunoblotting
Immunoblot analysis of gp91phox in macrophage cell lysates at 18 h pi [a], normalized to β-actin loading control [b]. ...View More
Koo, S. J., Szczesny, B., Wan, X., Putluri, N., & Garg, N. J. (2018). Pentose phosphate shunt modulates reactive oxygen species and nitric oxide production controlling Trypanosoma cruzi in macrophages. Frontiers in immunology, 9, 202. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
NL7
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P, FC

Basic Information

Immunogen
Purified gp91-phox of human origin, with epitope mapping to amino acids 498-507.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CYBB
Entrez Gene ID
Human1536
Mouse13058
Rat66021
UniProt ID
HumanP04839
MouseQ61093
RatQ9ERL1
Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
Cell redox homeostasis Source: Reactome
Cellular response to cadmium ion Source: Ensembl
Cellular response to ethanol Source: Ensembl
Cellular response to L-glutamine Source: Ensembl
Cellular response to oxidative stress Source: Reactome
Defense response Source: GO_Central
Electron transport chain Source: GOC
Hypoxia-inducible factor-1alpha signaling pathway Source: Ensembl
Inflammatory response Source: UniProtKB
Innate immune response Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of tumor necrosis factor production Source: Ensembl
Regulation of ion transmembrane transport Source: UniProtKB-KW
Respiratory burst Source: BHF-UCL
Response to aldosterone Source: Ensembl
Response to angiotensin Source: Ensembl
Response to drug Source: Ensembl
Response to nutrient Source: Ensembl
Superoxide anion generation Source: BHF-UCL
Superoxide metabolic process Source: BHF-UCL
Vascular endothelial growth factor receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane. As unassembled monomer may localize to the endoplasmic reticulum.
Involvement in disease
Granulomatous disease, chronic, X-linked (CGD):
A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.
Immunodeficiency 34 (IMD34):
A form of Mendelian susceptibility to mycobacterial disease, a rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals.
Topology
Cytoplasmic: 2-8
Helical: 9-29
Extracellular: 30-48
Helical: 49-69
Cytoplasmic: 70-102
Helical: 103-123
Extracellular: 124-169
Helical: 170-190
Cytoplasmic: 191-200
Helical: 201-221
Extracellular: 222-261
Helical: 262-282
Cytoplasmic: 283-570
PTM
Glycosylated.
Phosphorylated on Ser and Thr residues.
Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CYBB Recombinant Antibody (NL7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry