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Rabbit Anti-CUL2 Recombinant Antibody (CBYY-C2339) (V2LY-0125-LY524)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBYY-C2339
Antibody Isotype
IgG
Application
WB, IP, IHC-P

Basic Information

Immunogen
Synthetic peptide within n-terminal human cullin 2.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IP1:10-1:50
IHC-P1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA, Glycerol & TBS
Preservative
Sodium Azide
Concentration
1 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cullin 2
Entrez Gene ID
Human8453
Mouse71745
Rat361258
UniProt ID
HumanQ13617
MouseQ9D4H8
RatD4A0H4
Function
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346).

May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Biological Process
G1/S transition of mitotic cell cycle Source: ProtInc
Intrinsic apoptotic signaling pathway Source: ProtInc
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Viral process Source: UniProtKB-KW
Cellular Location
Cytosol; Nucleoplasm; Cul2-RING ubiquitin ligase complex; Cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; VCB complex
PTM
Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).
More Infomation

Diaz, S., Li, L., Wang, K., & Liu, X. (2021). Expression and purification of functional recombinant CUL2• RBX1 from E. coli. Scientific reports, 11(1), 1-10.

Chu, D., Liu, T., Yao, Y., & Luan, N. (2021). LINC00997/MicroRNA 574-3p/CUL2 Promotes Cervical Cancer Development via Mitogen-Activated Protein Kinase Signaling. Molecular and Cellular Biology, 41(8), e00059-21.

Ji, Z., Wang, X., Liu, Y., Zhong, M., Sun, J., & Shang, J. (2021). MicroRNA-574-3p Regulates HIF-α Isoforms Promoting Gastric Cancer Epithelial-Mesenchymal Transition via Targeting CUL2. Digestive diseases and sciences, 1-11.

Zhao, W., Liu, Y., Zhang, L., Ding, L., Li, Y., Zhang, H., ... & Hao, M. (2020). MicroRNA-154-5p regulates the HPV16 E7-pRb pathway in Cervical Carcinogenesis by targeting CUL2. Journal of Cancer, 11(18), 5379.

Faull, S. V., Lau, A., Martens, C., Ahdash, Z., Hansen, K., Yebenes, H., ... & Politis, A. (2019). Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome. Nature communications, 10(1), 1-13.

Odon, V., Georgana, I., Holley, J., Morata, J., & Maluquer de Motes, C. (2018). Novel class of viral ankyrin proteins targeting the host E3 ubiquitin ligase Cullin-2. Journal of Virology, 92(23), e01374-18.

Westrich, J. A., Warren, C. J., Klausner, M. J., Guo, K., Liu, C. W., Santiago, M. L., & Pyeon, D. (2018). Human papillomavirus 16 E7 stabilizes APOBEC3A protein by inhibiting cullin 2-dependent protein degradation. Journal of virology, 92(7), e01318-17.

Michail, O., Moris, D., Theocharis, S., & Griniatsos, J. (2018). Cullin-1 and-2 protein expression in colorectal cancer: correlation with clinicopathological variables. in vivo, 32(2), 391-396.

Cardote, T. A., Gadd, M. S., & Ciulli, A. (2017). Crystal structure of the Cul2-Rbx1-EloBC-VHL ubiquitin ligase complex. Structure, 25(6), 901-911.

Cardote, T. A., & Ciulli, A. (2017). Structure‐Guided Design of Peptides as Tools to Probe the Protein–Protein Interaction between Cullin‐2 and Elongin BC Substrate Adaptor in Cullin RING E3 Ubiquitin Ligases. ChemMedChem, 12(18), 1491-1496.

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For research use only. Not intended for any clinical use.

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