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Mouse Anti-CSRNP1 Recombinant Antibody (CBXC-0594) (V2LY-0125-LY80)


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Published Data
V2LY-0125-LY80-1
Mouse Anti-CSRNP1 Recombinant Antibody (CBXC-0594) (V2LY-0125-LY80) in WB
IL-1+OSM induces nuclear expression of CSRNP1.
Human chondrocytes were stimulated with IL-1 (0.05 ng/mL) in combination with OSM (10 ng/mL) for the indicated durations. Protein from either whole cell lysates (A) or subcellular fractions (C: a, cytosolic; b, membrane-bound; c, soluble nuclear; d, chromatin-bound; e, cytoskeletal) were resolved using SDS-PAGE and immunoblotted with an antibody to CSRNP1. Combined densitometric scans of separate blots for CSRNP1 in cell lysates (n = 3; B) or pooled soluble and chromatin-bound nuclear fractions (n = 5; D), relative to t = 0 h, are shown and were obtained from separate chondrocyte populations. ...View More
Macdonald, C. D., Falconer, A. M., Chan, C. M., Wilkinson, D. J., Skelton, A., Reynard, L., ... & Rowan, A. D. (2018). Cytokine-induced cysteine-serine-rich nuclear protein-1 (CSRNP1) selectively contributes to MMP1 expression in human chondrocytes. PLoS One, 13(11), e0207240. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY80-2
Mouse Anti-CSRNP1 Recombinant Antibody (CBXC-0594) (V2LY-0125-LY80) in WB
CSRNP1 selectively binds the proximal MMP1 AP-1 motif at a transcriptionally active time-point in IL-1+OSM-stimulated chondrocytes.
Human chondrocytes were treated with IL-1 (0.2 ng/mL) in combination with OSM (10 ng/mL) for 3 h. Nuclear lysates were prepared and subjected to either Western blotting alone (input) or DAPA with CSRNP1 or ATF3 antibodies, as described in the Materials and Methods. Non-biotinylated AP-1 oligonucleotides (50x excess; Comp) were used to confirm specificity of binding in the DAPAs. ...View More
Macdonald, C. D., Falconer, A. M., Chan, C. M., Wilkinson, D. J., Skelton, A., Reynard, L., ... & Rowan, A. D. (2018). Cytokine-induced cysteine-serine-rich nuclear protein-1 (CSRNP1) selectively contributes to MMP1 expression in human chondrocytes. PLoS One, 13(11), e0207240. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-0594
Antibody Isotype
IgG1
Application
WB, IP

Basic Information

Immunogen
Recombinant protein corresponding to the N-terminal region of human AXUD1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.1 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cysteine And Serine Rich Nuclear Protein 1
Entrez Gene ID
64651
UniProt ID
Q96S65
Function
Binds to the consensus sequence 5'-AGAGTG-3' and has transcriptional activator activity (By similarity).

May have a tumor-suppressor function. May play a role in apoptosis.
Biological Process
Apoptotic process Source: UniProtKB
Face morphogenesis Source: Ensembl
Platelet-derived growth factor receptor signaling pathway Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Post-embryonic development Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Roof of mouth development Source: Ensembl
Skeletal system morphogenesis Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
SRC kinase activity has been shown to be increased in several tumor tissues and tumor cell lines such as colon carcinoma cells.
Thrombocytopenia 6 (THC6):
A form of thrombocytopenia, a hematologic disorder defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. THC6 is an autosomal dominant form. Affected individuals may also have bone abnormalities and an increased risk for myelofibrosis.
PTM
Myristoylated at Gly-2, and this is essential for targeting to membranes.
Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation. CDK5-mediated phosphorylation at Ser-75 targets SRC to ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. Phosphorylated by PTK2/FAK1; this enhances kinase activity. Phosphorylated by PTK2B/PYK2; this enhances kinase activity. Upon activation of IL6ST by IL6, Tyr-419 is phosphorylated and Tyr-530 dephosphorylated (PubMed:25731159).
S-nitrosylation is important for activation of its kinase activity.
Ubiquitinated in response to CDK5-mediated phosphorylation. Ubiquitination mediated by CBLC requires SRC autophosphorylation at Tyr-419 and may lead to lysosomal degradation.
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For research use only. Not intended for any clinical use.

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