Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CSK Recombinant Antibody (CBXC-3040) (V2LY-1206-LY1504)


See all CSK antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBXC-3040
Antibody Isotype
IgG2b
Application
WB, IF

Basic Information

Immunogen
CSK Fusion Protein Ag12103 (83-450 aa).
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IF(ICC)1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
C-Terminal Src Kinase
Entrez Gene ID
Human1445
Mouse12988
UniProt ID
HumanP41240
MouseP41241
Function
Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Adherens junction organization Source: GO_Central
Brain development Source: Ensembl
Cellular response to peptide hormone stimulus Source: Ensembl
Cytokine-mediated signaling pathway Source: Reactome
Negative regulation of bone resorption Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of Golgi to plasma membrane protein transport Source: UniProtKB
Negative regulation of interleukin-6 production Source: Ensembl
Negative regulation of kinase activity Source: Ensembl
Negative regulation of low-density lipoprotein particle clearance Source: Ensembl
Negative regulation of phagocytosis Source: Ensembl
Oligodendrocyte differentiation Source: Ensembl
Positive regulation of MAP kinase activity Source: Ensembl
Protein autophosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Regulation of Fc receptor mediated stimulatory signaling pathway Source: GO_Central
T cell costimulation Source: Reactome
T cell receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane; Cytoplasm. Mainly cytoplasmic, also present in lipid rafts.
PTM
Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.
More Infomation

Xiao, L., Salem, J. E., Clauss, S., Hanley, A., Bapat, A., Hulsmans, M., ... & Milan, D. J. (2020). Ibrutinib-mediated atrial fibrillation attributable to inhibition of C-terminal Src kinase. Circulation, 142(25), 2443-2455.

P O’Malley, D. (2020). Recent advances in inhibitors of C-terminal SRC kinase. Future Medicinal Chemistry, 12(16), 1447-1449.

Simoncelli, S., Griffié, J., Williamson, D. J., Bibby, J., Bray, C., Zamoyska, R., ... & Owen, D. M. (2020). Multi-color molecular visualization of signaling proteins reveals how C-terminal Src kinase nanoclusters regulate T cell receptor activation. Cell Reports, 33(12), 108523.

O’Malley, D. P., Ahuja, V., Fink, B., Cao, C., Wang, C., Swanson, J., ... & Xie, D. (2019). Discovery of pyridazinone and pyrazolo [1, 5-a] pyridine inhibitors of C-terminal Src kinase. ACS medicinal chemistry letters, 10(10), 1486-1491.

Samarasekera, G. G., & Auld, V. J. (2018). C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. Molecular biology of the cell, 29(2), 123-136.

Potuckova, L., Draberova, L., Halova, I., Paulenda, T., & Draber, P. (2018). Positive and negative regulatory roles of C-terminal Src kinase (CSK) in FcεRI-mediated mast cell activation, independent of the transmembrane adaptor PAG/CSK-binding protein. Frontiers in immunology, 9, 1771.

Berman-Booty, L. D., Eraslan, R., Hanumegowda, U., Cantor, G. H., Bounous, D. I., Janovitz, E. B., ... & Wee, S. (2018). Systemic loss of C-terminal Src kinase expression elicits spontaneous suppurative inflammation in conditional knockout mice. Veterinary pathology, 55(2), 331-340.

Taskinen, B., Ferrada, E., & Fowler, D. M. (2017). Early emergence of negative regulation of the tyrosine kinase Src by the C-terminal Src kinase. Journal of Biological Chemistry, 292(45), 18518-18529.

Liu, D., Yuan, Y., Xu, R., & Cowburn, D. (2017). Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling. Protein & cell, 8(1), 67-71.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CSK Recombinant Antibody (CBXC-3040)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry