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Mouse Anti-CPSF4 Recombinant Antibody (CBXC-1788) (V2LY-1206-LY992)


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Published Data
V2LY-1206-LY992-1
Mouse Anti-CPSF4 Recombinant Antibody (CBXC-1788) (V2LY-1206-LY992) in WB
Control of expression of recombinant CPSF30 protein and deletion mutants. Cells were transfected with pcDNA3 expression vectors encoding 1xmyc-CPSF4, 5xmyc-f2f3 (CPSF30), 5xmyc-f2f3 mutant (CPSF30), and EGFP. Protein lysates were analyzed by Western blotting. ...View More
Nacken, W., Schreiber, A., Masemann, D., & Ludwig, S. (2021). The effector domain of the influenza A virus nonstructural protein NS1 triggers host shutoff by mediating inhibition and global deregulation of host transcription when associated with specific structures in the nucleus. MBio, 12(5), 10-1128.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Pig, Horse
Clone
CBXC-1788
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-102 mapping at the N-terminus of human CPSF4.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Pig, Horse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cleavage And Polyadenylation Specific Factor 4
Entrez Gene ID
Human10898
Mouse54188
Rat304277
UniProt ID
HumanO95639
MouseQ8BQZ5
RatQ5FVR7
Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Biological Process
modification by virus of host mRNA processing Source: Reactome
mRNA 3'-end processing Source: Reactome
mRNA export from nucleus Source: Reactome
mRNA splicing, via spliceosome Source: Reactome
Pre-mRNA cleavage required for polyadenylation Source: GO_Central
Termination of RNA polymerase II transcription Source: Reactome
tRNA splicing, via endonucleolytic cleavage and ligation Source: Reactome
Cellular Location
Nucleus
More Infomation

Wang, X., Dong, J., Li, X., Cheng, Z., & Zhu, Q. (2021). CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. Oncogene, 40(25), 4338-4351.

Lee, K., Zheng, Q., Lu, Q., Xu, F., Qin, G., Zhai, Q., ... & Wang, S. (2021). CPSF4 promotes triple negative breast cancer metastasis by upregulating MDM4. Signal transduction and targeted therapy, 6(1), 1-3.

Zhang, M., Lin, H., Ge, X., & Xu, Y. (2021). Overproduced CPSF4 promotes cell proliferation and invasion via PI3K-AKT signaling pathway in oral squamous cell carcinoma. Journal of Oral and Maxillofacial Surgery, 79(5), 1177-e1.

Wang, X., Jiang, G., Ren, W., Wang, B., Yang, C., & Li, M. (2020). LncRNA NEAT1 regulates 5-Fu sensitivity, apoptosis and invasion in colorectal cancer through the MiR-150-5p/CPSF4 axis. OncoTargets and therapy, 13, 6373.

Yang, Q., Fan, W., Zheng, Z., Lin, S., Liu, C., Wang, R., ... & Wu, X. (2019). Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(10), 1533-1543.

Wu, J., Miao, J., Ding, Y., Zhang, Y., Huang, X., Zhou, X., & Tang, R. (2019). MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4. Biochemistry and Cell Biology, 97(6), 722-730.

Dubois, J., Traversier, A., Julien, T., Padey, B., Lina, B., Bourdon, J. C., ... & Terrier, O. (2019). The nonstructural NS1 protein of influenza viruses modulates TP53 splicing through host factor CPSF4. Journal of virology, 93(7), e02168-18.

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For research use only. Not intended for any clinical use.

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