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Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986)


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Published Data
V2LY-1206-LY986-1
Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986) in WB
Whole cell lysates from THP-1 cells treated with PMA for 0, 1, 6, 18 and 24 hours were separated by 10% SDS-PAGE and western blotting was performed for the indicated subunits of the C/P complex, with β-actin as the loading control. Each western blot (left panel) was performed in three biological replicates of the differentiation process and the quantified data is shown in the right panel. ...View More
Mukherjee, S., Graber, J. H., & Moore, C. L. (2023). Macrophage differentiation is marked by increased abundance of the mRNA 3'end processing machinery, altered poly (A) site usage, and sensitivity to the level of CstF64. Frontiers in immunology, 14, 1091403.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY986-2
Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986) in WB
ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes.
Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against (A) p80 and p62 of TFIIH, (B) CPSF2 and CPSF7 of the cleavage and polyadenylation complex and (C) Brahma of Swi/Snf, CHD3 of the Nurd complex, and RuvBL1 and RuvBL2 of the Ino80 complex were used. ...View More
Wagner, L. M., & DeLuca, N. A. (2013). Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription. PloS one, 8(10), e78242.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Horse
Clone
CBXC-1743
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 258-295 within an internal region of human CPSF2.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Horse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cleavage And Polyadenylation Specific Factor 2
Entrez Gene ID
Human53981
Mouse51786
Rat299256
UniProt ID
HumanQ9P2I0
MouseO35218
RatA6JEJ7
Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
Biological Process
mRNA 3'-end processing Source: Reactome
mRNA 3'-end processing by stem-loop binding and cleavage Source: UniProtKB
mRNA export from nucleus Source: Reactome
mRNA polyadenylation Source: GO_Central
mRNA splicing, via spliceosome Source: Reactome
Pre-mRNA cleavage required for polyadenylation Source: GO_Central
Termination of RNA polymerase II transcription Source: Reactome
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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