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Mouse Anti-CLK1 Recombinant Antibody (CBWJC-2808) (V2LY-1206-LY244)

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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJC-2808
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 202-227 within an internal region of CLK1 of human.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CDC Like Kinase 1
Entrez Gene ID
UniProt ID
Function
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
Biological Process
Peptidyl-serine phosphorylation Source: Ensembl
Peptidyl-threonine phosphorylation Source: Ensembl
Protein autophosphorylation Source: Ensembl
Regulation of RNA splicing Source: UniProtKB
Cellular Location
Nucleus
PTM
Autophosphorylates on all three types of residues.
More Infomation

Dekel, N., Eisenberg-Domovich, Y., Karlas, A., Meyer, T. F., Bracher, F., Lebendiker, M., ... & Livnah, O. (2020). Expression, purification and crystallization of CLK1 kinase–A potential target for antiviral therapy. Protein Expression and Purification, 176, 105742.

Babu, N., Pinto, S. M., Biswas, M., Subbannayya, T., Rajappa, M., Mohan, S. V., ... & Chatterjee, A. (2020). Phosphoproteomic analysis identifies CLK1 as a novel therapeutic target in gastric cancer. Gastric Cancer, 23(5), 796-810.

Artarini, A., Meyer, M., Shin, Y. J., Huber, K., Hilz, N., Bracher, F., ... & Karlas, A. (2019). Regulation of influenza A virus mRNA splicing by CLK1. Antiviral research, 168, 187-196.

Sun, L., Cheong, J. E., Zaffagni, M., Zhou, K., & Zetter, B. (2018). Discovery of novel water-soluble derivatives of mebendazole as selective CLK1/4 kinase inhibitors and their anticancer cancer activity.

Uzor, S., Zorzou, P., Bowler, E., Porazinski, S., Wilson, I., & Ladomery, M. (2018). Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention. Gene, 670, 46-54.

Sako, Y., Ninomiya, K., Okuno, Y., Toyomoto, M., Nishida, A., Koike, Y., ... & Hagiwara, M. (2017). Development of an orally available inhibitor of CLK1 for skipping a mutated dystrophin exon in Duchenne muscular dystrophy. Scientific reports, 7(1), 1-9.

Sun, Q. Z., Lin, G. F., Li, L. L., Jin, X. T., Huang, L. Y., Zhang, G., ... & Yang, S. Y. (2017). Discovery of potent and selective inhibitors of cdc2-like kinase 1 (clk1) as a new class of autophagy inducers. Journal of medicinal chemistry, 60(14), 6337-6352.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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