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Rabbit Anti-CKAP4 Recombinant Antibody (C3827) (CBMAB-C3827WJ)


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Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
C3827
Antibody Isotype
IgG
Application
WB, IF, IHC-P

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human CKAP4.
Host Species
Rabbit
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4
Preservative
None
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytoskeleton Associated Protein 4
Introduction
CKAP4 (Cytoskeleton Associated Protein 4) is a Protein Coding gene. Diseases associated with CKAP4 include Cystitis and Epispadias. Among its related pathways are Innate Immune System and Protein processing in endoplasmic reticulum. Gene Ontology (GO) annotations related to this gene include signal transducer activity.
Entrez Gene ID
Human10970
Monkey702779
UniProt ID
HumanQ07065
MonkeyA0A2K5TSL3
Alternative Names
Cytoskeleton Associated Protein 4; 3-KDa Cytoskeleton-Linking Membrane Protein; Cytoskeleton-Associated Protein 4; CLIMP-63; P63; Transmembrane Protein (63kD), Endoplasmic Reticulum/Golgi Intermediate Compartment; Type-II Transmembrane Protein P63; 3 KDa Membrane Protein; ERGIC-63;
Function
Mediates the anchoring of the endoplasmic reticulum to microtubules.

High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.
Biological Process
Cellular protein metabolic process Source: Reactome
Neutrophil degranulation Source: Reactome
Post-translational protein modification Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Cytoskeleton; Cell membrane; Perinuclear region. Translocates to the perinuclear region upon APF-stimulation.
Topology
Cytoplasmic: 1-106
Helical: 107-127
Extracellular: 128-602
PTM
Reversibly palmitoylated. Palmitoylation at Cys-100 by ZDHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization. Palmitoylation by ZDHHC2 is also required for its function in APF-mediated antiproliferative signaling (PubMed:19144824).
Increased phosphorylation during mitosis prevents binding to microtubules.
More Infomation

Luo, T., Ding, K., Ji, J., Zhang, X., Yang, X., Chen, A., ... & Li, X. (2021). Cytoskeleton-associated protein 4 (CKAP4) promotes malignant progression of human gliomas through inhibition of the Hippo signaling pathway. Journal of Neuro-Oncology, 154(3), 275-283.

Wang, Y., Yu, H., Xie, X., Deng, T., Ye, L., Wu, L., ... & Chen, G. (2021). Plasmalemma vesicle-associated protein promotes angiogenesis in cholangiocarcinoma via the DKK1/CKAP4/PI3K signaling pathway. Oncogene, 1-14.

Li, S. X., Li, J., Dong, L. W., & Guo, Z. Y. (2020). Cytoskeleton-associated protein 4, a promising biomarker for tumor diagnosis and therapy. Frontiers in Molecular Biosciences, 7.

Chen, Z. Y., Wang, T., Gan, X., Chen, S. H., He, Y. T., Wang, Y. Q., & Zhang, K. H. (2020). Cytoskeleton-associated membrane protein 4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma. Oncology letters, 19(6), 3889-3898.

Wang, Y., Yu, W., He, M., Huang, Y., Wang, M., & Zhu, J. (2019). Serum cytoskeleton-associated protein 4 as a biomarker for the diagnosis of hepatocellular carcinoma. OncoTargets and therapy, 12, 359.

Chen, L., You, C., Jin, X., Zhou, L., Huang, L., & Wang, Y. (2018). Cytoskeleton-associated protein 4 is a novel serodiagnostic marker for esophageal squamous-cell carcinoma. OncoTargets and therapy, 11, 8221.

Yanagita, K., Nagashio, R., Jiang, S. X., Kuchitsu, Y., Hachimura, K., Ichinoe, M., ... & Sato, Y. (2018). Cytoskeleton-associated protein 4 is a novel serodiagnostic marker for lung cancer. The American journal of pathology, 188(6), 1328-1333.

Gladka, M. M., Molenaar, B., De Ruiter, H., Van Der Elst, S., Tsui, H., Versteeg, D., ... & Van Rooij, E. (2018). Single-cell sequencing of the healthy and diseased heart reveals cytoskeleton-associated protein 4 as a new modulator of fibroblasts activation. Circulation, 138(2), 166-180.

Kikuchi, A., Fumoto, K., & Kimura, H. (2017). The Dickkopf1‐cytoskeleton‐associated protein 4 axis creates a novel signalling pathway and may represent a molecular target for cancer therapy. British journal of pharmacology, 174(24), 4651-4665.

Chavda, B., Ling, J., Majernick, T., & Planey, S. L. (2017). Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain. BMC biochemistry, 18(1), 1-14.

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For research use only. Not intended for any clinical use.

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