Mouse Anti-CHST12 Recombinant Antibody (CBXC-0424) (CBMAB-C5787-CQ)

Name: Mouse Anti-CHST12 Recombinant Antibody (CBXC-0424)
SKU: CBMAB-C5787-CQ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBXC-0424

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

CHST12 (56 a.a. ~ 150 a.a) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

carbohydrate (chondroitin 4) sulfotransferase 12

Introduction

The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene.

Entrez Gene ID

55501

UniProt ID

Q9NRB3

Alternative Names

Carbohydrate Sulfotransferase 12; Chondroitin 4-Sulfotransferase 2; Carbohydrate (Chondroitin 4) Sulfotransferase 12; Chondroitin 4-O-Sulfotransferase 2; Sulfotransferase Hlo; C4ST-2; C4ST2; EC 2.8.2.5; C4S-2;

Function

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

Biological Process

Carbohydrate biosynthetic process Source: InterPro
Chondroitin sulfate biosynthetic process Source: UniProtKB
Dermatan sulfate biosynthetic process Source: UniProtKB
Proteoglycan biosynthetic process Source: GO_Central

Cellular Location

Golgi apparatus membrane

Topology

Cytoplasmic: 1-5
Helical: 6-26
Lumenal: 27-414

More Infomation

References

Wang, J., Xia, X., Tao, X., Zhao, P., & Deng, C. (2021). Knockdown of carbohydrate sulfotransferase 12 decreases the proliferation and mobility of glioblastoma cells via the WNT/β-catenin pathway. Bioengineered, 12(1), 3934-3946.

Guo, Y., Zhou, Y., Yan, S., Qu, C., Wang, L., Guo, X., & Han, J. (2019). Decreased expression of CHST-12, CHST-13, and UST in the proximal interphalangeal joint cartilage of school-age children with Kashin–Beck disease: an endemic osteoarthritis in China caused by selenium deficiency. Biological trace element research, 191(2), 276-285.

Wang, L., Guo, X., Yi, J., Qu, C., Lei, J., Guo, Q., & Han, J. (2018). The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats. Journal of Trace Elements in Medicine and Biology, 50, 123-129.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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