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Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY)


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Published Data
CBMAB-C9161-LY-1
Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY) in WB
Quantitative analysis of α9-containing nAChRs by Western blot in various cancer cell lines and breast epithelial cell lines. GAPDH was the control in all experiments. The proteins were detected with the corresponding antibody and an anti-mouse IgG secondary antibody conjugated to horseradish peroxidase (HRP). Each bar is the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, breast cancer cell lines vs. breast epithelial cell lines HS578BST (normal cell control). ...View More
Sun, Z., Zhangsun, M., Dong, S., Liu, Y., Qian, J., Zhangsun, D., & Luo, S. (2020). Differential expression of nicotine acetylcholine receptors associates with human breast cancer and mediates antitumor activity of αO-conotoxin gexiva. Marine drugs, 18(1), 61.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C9161-LY-2
Mouse Anti-CHRNA9 Recombinant Antibody (8E4) (CBMAB-C9161-LY) in WB
Western blot images of α3, α4, α5, α6, α7, α9, α10, β2, β3, and β4 nAChR. ...View More
Liu, Y., Qian, J., Sun, Z., Zhangsun, D., & Luo, S. (2019). Cervical cancer correlates with the differential expression of nicotinic acetylcholine receptors and reveals therapeutic targets. Marine drugs, 17(5), 256.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
8E4
Antibody Isotype
IgG2a, κ
Application
ELISA, WB, IP

Basic Information

Immunogen
Amino acids 139-221 of AChRα9 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cholinergic receptor, nicotinic, alpha 9
Introduction
This gene is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. [provided by RefSeq, Feb 2012]
Entrez Gene ID
55584
UniProt ID
Q9UGM1
Alternative Names
Cholinergic Receptor Nicotinic Alpha 9 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 9; Cholinergic Receptor, Nicotinic, Alpha 9 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 9; NACHRA9; Acetylcholine Receptor, Neuronal Nicotinic, Alpha-
Function
Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane (PubMed:11752216, PubMed:25282151).
The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane (PubMed:11752216, PubMed:25282151).
In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion (PubMed:11021840).
Biological Process
Chemical synaptic transmission Source: GO_Central
Detection of mechanical stimulus involved in sensory perception of sound Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Ion transmembrane transport Source: GO_Central
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Nervous system process Source: GO_Central
Positive regulation of cytosolic calcium ion concentration Source: MGI
Regulation of membrane potential Source: GO_Central
Signal transduction Source: GO_Central
Cellular Location
Postsynaptic cell membrane; Cell membrane
Topology
Extracellular: 26-237
Helical: 238-262
Helical: 269-287
Helical: 302-323
Cytoplasmic: 324-457
Helical: 458-476
PTM
N-glycosylated.
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For research use only. Not intended for any clinical use.

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