Mouse Anti-CHRNA3 Recombinant Antibody (0072) (CBMAB-0072-CN)

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Published Data

Mouse Anti-CHRNA3 Recombinant Antibody (0072) (CBMAB-0072-CN) in WB

nAChR subunits expression measured by Western blot in human spermatozoa (PC = Positive control from mouse brain homogenate; HS = Human spermatozoa). ...View More

Condorelli, R. A., La Vignera, S., Giacone, F., Iacoviello, L., Mongioì, L. M., Li Volti, G., ... & Calogero, A. E. (2017). Nicotine effects and receptor expression on human spermatozoa: Possible neuroendocrine mechanism. Frontiers in Physiology, 8, 177.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

0072

Antibody Isotype

IgG2b, κ

Application

ELISA, IF, WB, IP

Basic Information

Immunogen

Amino acids 346-445 mapping near the C-terminus of AChRα3 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

CHRNA3

Introduction

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Entrez Gene ID

Human	1136
Rat	25101

UniProt ID

Human	P32297
Rat	P04757

Alternative Names

LNCR2; PAOD2; NACHRA3

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Acetylcholine receptor signaling pathway Source: ARUK-UCL
Activation of transmembrane receptor protein tyrosine kinase activity Source: UniProtKB
Behavioral response to nicotine Source: UniProtKB
Chemical synaptic transmission Source: GO_Central
Excitatory postsynaptic potential Source: UniProtKB
Ion transmembrane transport Source: GO_Central
Ion transport Source: UniProtKB
Locomotory behavior Source: UniProtKB
Nervous system development Source: UniProtKB
Nervous system process Source: GO_Central
Regulation of acetylcholine secretion, neurotransmission Source: UniProtKB
Regulation of dendrite morphogenesis Source: UniProtKB
Regulation of membrane potential Source: UniProtKB
Regulation of smooth muscle contraction Source: UniProtKB
Response to acetylcholine Source: ARUK-UCL
Signal transduction Source: UniProtKB
Synaptic transmission, cholinergic Source: UniProtKB
Synaptic transmission involved in micturition Source: UniProtKB

Cellular Location

Postsynaptic cell membrane; Cell membrane

Involvement in disease

Bladder dysfunction, autonomic, with impaired pupillary reflex and secondary CAKUT (BAIPRCK): An autosomal recessive disease characterized by impaired innervation and autonomic dysfunction of the urinary bladder, hydronephrosis, vesicoureteral reflux, small kidneys, recurrent urinary tract infections, and progressive renal insufficiency. Additional autonomic features are impaired pupillary reflex and orthostatic hypotension. The disease manifests in utero or early childhood.

Topology

Extracellular: 32-240
Helical: 241-265
Helical: 273-291
Helical: 307-328
Cytoplasmic: 329-477
Helical: 478-497

More Infomation

References

Shibao, C., Joos, K., Phillips, J. A., Cogan, J., Newman, J. H., Hamid, R., ... & Biaggioni, I. (2021). Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants. Neurology.

Wu, T., Wang, Y., Shi, W., Zhang, B. Q., Raelson, J., Yao, Y. M., ... & Wu, J. (2020). A variant in the nicotinic acetylcholine receptor alpha 3 subunit gene is associated with hypertension risks in hypogonadic patients. Frontiers in genetics, 11, 1526.

Yang, X., Guo, X., Huang, Z., Da, Y., Xing, W., Li, F., ... & Zhu, Y. (2019). CHRNA5/CHRNA3 gene cluster is a risk factor for lumbar disc herniation: a case-control study. Journal of orthopaedic surgery and research, 14(1), 1-7.

Lee, S. H., Barrie, E. S., Sadee, W., & Smith, R. M. (2019). Nicotine Dependence and the CHRNA5/CHRNA3/CHRNB4 Nicotinic Receptor Regulome. In Neuroscience of Nicotine (pp. 347-353). Academic Press.

Shibao, C. A., Phillips, J., Cogan, J. D., Newman, J. H., Hamid, R., Meiler, J., ... & Biaggioni, I. (2019). Abstract P176: Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants. Novel Genetic Cause of Neurogenic Orthostatic Hypotension. Hypertension, 74(Suppl_1), AP176-AP176.

Shibao, C., Phillips, J. A., Cogan, J. D., Newman, J. H., Hamid, R., Sheehan, J., ... & Biaggioni, I. (2018). Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic Variants: Novel Genetic Cause of Neurogenic Orthostatic Hypotension. Available at SSRN 3260799.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CHRNA3 Recombinant Antibody (CBLNC-158)

Mouse Anti-CHRNA3 Recombinant Antibody (0265)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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