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Rabbit Anti-CHRM2 Recombinant Antibody (EG682) (CBMAB-EN823-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG682
Antibody Isotype
IgG
Application
WB, IF, IHC, FC

Basic Information

Immunogen
Synthetic peptide within Human Muscarinic Acetylcholine Receptor M2/CHRM2 aa 140-184.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IHC1:1,000
IF(ICC)1:100
FC1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, pH7.4, 0.05% BSA, 40% Glycerol
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cholinergic Receptor Muscarinic 2
Introduction
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human1129
Mouse243764
Rat81645
UniProt ID
HumanP08172
MouseQ9ERZ4
RatP10980
Alternative Names
Cholinergic Receptor Muscarinic 2; Acetylcholine Receptor, Muscarinic 2; Muscarinic Acetylcholine Receptor M2; Cholinergic Receptor, Muscarinic 2; Muscarinic M2 Receptor; 7TM Receptor; HM2;
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Biological Process
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Chemical synaptic transmission Source: GO_Central
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Membrane organization Source: Reactome
Nervous system development Source: ProtInc
Phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway Source: ProtInc
Regulation of heart contraction Source: ProtInc
Regulation of smooth muscle contraction Source: GO_Central
Response to virus Source: UniProtKB
Cellular Location
Cell membrane; Postsynaptic cell membrane. Phosphorylation in response to agonist binding promotes receptor internalization.
Involvement in disease
Major depressive disorder (MDD): A common psychiatric disorder. It is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
Topology
Extracellular: 1-22
Helical: 23-45
Cytoplasmic: 46-59
Helical: 60-80
Extracellular: 81-97
Helical: 98-119
Cytoplasmic: 120-139
Helical: 140-162
Extracellular: 163-184
Helical: 185-209
Cytoplasmic: 210-387
Helical: 388-410
Extracellular: 411-418
Helical: 419-442
Cytoplasmic: 443-466
PTM
Phosphorylated in response to agonist treatment.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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