Mouse Anti-CHMP5 Recombinant Antibody (CBYY-C3073) (CBMAB-C4516-YY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Cattle, Horse

Clone

CBYY-C3073

Antibody Isotype

IgG2b, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 130-219 mapping at the C-terminus of CHMP5 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Horse

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Charged Multivesicular Body Protein 5

Entrez Gene ID

51510

UniProt ID

Q9NZZ3

Alternative Names

Cyclin A2; Cyclin-A; CCN1; CCNA; Cyclin-A2; Cyclin A;

Function

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.

Biological Process

Cellular response to lipopolysaccharide Source: UniProtKB
Cellular response to muramyl dipeptide Source: UniProtKB
Endosomal transport Source: Reactome
Endosome to lysosome transport Source: Ensembl
ESCRT III complex disassembly Source: ParkinsonsUK-UCL
Late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
Lysosome organization Source: Ensembl
Midbody abscission Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Multivesicular body assembly Source: ParkinsonsUK-UCL
Multivesicular body sorting pathway Source: UniProtKB
Nucleus organization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of centrosome duplication Source: UniProtKB
Regulation of mitotic spindle assembly Source: UniProtKB
Regulation of receptor recycling Source: Ensembl
Vesicle budding from membrane Source: GO_Central
Viral budding Source: UniProtKB
Viral life cycle Source: Reactome

Cellular Location

Endosome membrane; Cytosol. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.

PTM

ISGylated. Isgylation inhibits its interaction with VTA1.

More Infomation

References

Pashkova, N., Yu, L., Schnicker, N. J., Tseng, C. C., Gakhar, L., Katzmann, D. J., & Piper, R. C. (2021). Interactions of ubiquitin and CHMP5 with the V domain of HD-PTP reveals role for regulation of Vps4 ATPase. Molecular biology of the cell, 32(22), ar42.

Umphred-Wilson, K., & Adoro, S. (2020). Abstract LB-047: CHMP5 dependency is a therapeutic vulnerability of T cell acute lymphoblastic leukemia.

Son, F., Umphred-Wilson, K., Shim, J. H., & Adoro, S. (2019). Assessment of ESCRT protein CHMP5 activity on client protein ubiquitination by immunoprecipitation and Western blotting. In The ESCRT Complexes (pp. 219-226). Humana, New York, NY.

Mo, J. S., Han, S. H., Yun, K. J., & Chae, S. C. (2018). MicroRNA 429 regulates the expression of CHMP5 in the inflammatory colitis and colorectal cancer cells. Inflammation Research, 67(11), 985-996.

Adoro, S., Park, K. H., Bettigole, S. E., Lis, R., Shin, H. R., Seo, H., ... & Glimcher, L. H. (2017). Post-translational control of T cell development by the ESCRT protein CHMP5. Nature immunology, 18(7), 780-790.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CHMP5 Recombinant Antibody (V0025)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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