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Mouse Anti-CHMP5 Recombinant Antibody (CBYY-C3073) (CBMAB-C4516-YY)


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Published Data
CBMAB-C4516-YY-1
Mouse Anti-CHMP5 Recombinant Antibody (CBYY-C3073) (CBMAB-C4516-YY) in IF
ESCRT-III knockdown inhibits exosome biogenesis in non-acidic SC compartments, but does not change compartment identity. Panels a-f show basal wide-field fluorescence views of living SCs expressing YFP-Rab11 from its endogenous genomic location (yellow). SC outline approximated by dashed white circles. Acidic compartments are marked by LysoTracker® Red (magenta). Boxed non-acidic compartments are magnified in A'-F' Zoom. ...View More
Marie, P. P., Fan, S. J., Mason, J., Wells, A., Mendes, C. C., Wainwright, S. M., ... & Goberdhan, D. C. (2023). Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation. Journal of extracellular vesicles, 12(3), 12311.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C4516-YY-2
Mouse Anti-CHMP5 Recombinant Antibody (CBYY-C3073) (CBMAB-C4516-YY) in WB
Western blot analysis of putative exosome proteins from glutamine-depleted (0.15 mM) EV-secreting HCT116 cells transduced with a CHMP5 (shCHMP5) compared to a non-targeting (shNT) shRNA construct shows selective reduction in CHMP5 protein levels. The activity of mTORC1 was assessed via phosphorylation of S6 and 4E-BP1, using phospho-specific antibodies and a pan 4E-BP1 antibody. Bar chart shows protein levels normalised to tubulin. Data derived from three independent experiments. ...View More
Marie, P. P., Fan, S. J., Mason, J., Wells, A., Mendes, C. C., Wainwright, S. M., ... & Goberdhan, D. C. (2023). Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation. Journal of extracellular vesicles, 12(3), 12311.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle, Horse
Clone
CBYY-C3073
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 130-219 mapping at the C-terminus of CHMP5 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Cattle, Horse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Charged Multivesicular Body Protein 5
Entrez Gene ID
51510
UniProt ID
Q9NZZ3
Alternative Names
Cyclin A2; Cyclin-A; CCN1; CCNA; Cyclin-A2; Cyclin A;
Function
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
Biological Process
Cellular response to lipopolysaccharide Source: UniProtKB
Cellular response to muramyl dipeptide Source: UniProtKB
Endosomal transport Source: Reactome
Endosome to lysosome transport Source: Ensembl
ESCRT III complex disassembly Source: ParkinsonsUK-UCL
Late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
Lysosome organization Source: Ensembl
Midbody abscission Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Multivesicular body assembly Source: ParkinsonsUK-UCL
Multivesicular body sorting pathway Source: UniProtKB
Nucleus organization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of centrosome duplication Source: UniProtKB
Regulation of mitotic spindle assembly Source: UniProtKB
Regulation of receptor recycling Source: Ensembl
Vesicle budding from membrane Source: GO_Central
Viral budding Source: UniProtKB
Viral life cycle Source: Reactome
Cellular Location
Endosome membrane; Cytosol. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
PTM
ISGylated. Isgylation inhibits its interaction with VTA1.
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For research use only. Not intended for any clinical use.

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