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Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY)


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Published Data
CBMAB-C1698-FY-1
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in IHC
Immunohistochemical analysis was performed of antral follicles of ovaries from mice in the control (n = 5), POI (n = 4), DQ (n = 5), and POI+DQ (n = 4) groups. Cross-sections of ovaries were stained with an anti-p16 antibody. ...View More
Xu, Z., Takahashi, N., Harada, M., Kunitomi, C., Kusamoto, A., Koike, H., ... & Osuga, Y. (2023). The Role of Cellular Senescence in Cyclophosphamide-Induced Primary Ovarian Insufficiency. International Journal of Molecular Sciences, 24(24), 17193.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1698-FY-2
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in Immunoblot
Representative images of immunoblot against senescence markers p53, p21, and p16. ...View More
Maharajan, N., Lee, C. M., Vijayakumar, K. A., & Cho, G. W. (2023). Oxymatrine Improves Oxidative Stress-Induced Senescence in HT22 Cells and Mice via the Activation of AMP-Activated Protein Kinase. Antioxidants, 12(12), 2078.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1698-FY-3
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in WB
Doxorubicin (DOXO) promotes senescence and decreases the expression of sirtuins in H9C2 cells. H9C2 cells were incubated with 0.1 µM of DOXO for 24 and 32 h. Western blot analysis showing the expression of p16. ...View More
Foglio, E., D'Avorio, E., Vitiello, L., Masuelli, L., Bei, R., Pacifici, F., ... & Limana, F. (2023). Doxorubicin-Induced Cardiac Senescence Is Alleviated Following Treatment with Combined Polyphenols and Micronutrients through Enhancement in Mitophagy. Cells, 12(22), 2605.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1698-FY-4
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in WB
Protein levels of Glipr1and p16Ink4a in the livers of mice administrated with saline (n = 8) or doxorubicin (n = 7) were examined using western blot analysis. Left panels are representative images of the chemiluminescence and stain-free. ...View More
Doshida, Y., Hashimoto, S., Iwabuchi, S., Takino, Y., Ishiwata, T., Aigaki, T., & Ishigami, A. (2023). Single-cell RNA sequencing to detect age-associated genes that identify senescent cells in the liver of aged mice. Scientific Reports, 13(1), 14186.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1698-FY-5
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in IF
Double immunostaining and quantification of p16 positive astrocytes in cortex slides from LOAD patients. ...View More
Jiang, C. S., Rana, T., Jin, L. W., Farr, S. A., Morley, J. E., Qin, H., ... & Liu, R. M. (2023). Aging, plasminogen activator inhibitor 1, brain cell senescence, and Alzheimer's Disease. Aging and Disease, 14(2), 515.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CBMAB-C1698-FY-6
Mouse Anti-CDKN2A Recombinant Antibody (CBFYC-1637) (CBMAB-C1698-FY) in WB
Western blotting of senescence signaling mediators (p53, p16, and p21) and cell cycle markers (proliferating cell nuclear antigen (PCNA) and p-Rb) in Pink1+/+ and Pink1−/− mice at the age of 4 and 24 months. ...View More
Ha, M. H., Kim, M. S., An, H. J., Sung, M. J., Lee, Y. H., Yang, D. H., ... & Jeong, H. Y. (2023). PTEN-induced kinase 1 is associated with renal aging, via the cGAS-STING pathway. Aging Cell, 22(7), e13865.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1637
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 1-168 representing full length p16 INK4A of mouse.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin Dependent Kinase Inhibitor 2A
Introduction
Thus some aberration of the CDKN2A, CDK4 and RB1 genes appears to be almost obligatory in glioblastomas.
Entrez Gene ID
1029
UniProt ID
P42771
Alternative Names
Cyclin Dependent Kinase Inhibitor 2A; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase 4 Inhibitor A; Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumor Suppressor 1; Alternative Reading Frame; P16-INK4A; P16INK4A; P14ARF; CDKN2; CDK4I; MTS-1; MTS1; MLM
Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Biological Process
Cell cycle arrest Source: BHF-UCL
Cellular senescence Source: BHF-UCL
G1/S transition of mitotic cell cycle Source: BHF-UCL
Negative regulation of cell growth Source: BHF-UCL
Negative regulation of cell-matrix adhesion Source: BHF-UCL
Negative regulation of cell population proliferation Source: BHF-UCL
Negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
Negative regulation of G1/S transition of mitotic cell cycle Source: Reactome
Negative regulation of NF-kappaB transcription factor activity Source: BHF-UCL
Negative regulation of phosphorylation Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of cellular senescence Source: UniProtKB
Positive regulation of macrophage apoptotic process Source: BHF-UCL
Positive regulation of smooth muscle cell apoptotic process Source: BHF-UCL
Ras protein signal transduction Source: BHF-UCL
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Replicative senescence Source: BHF-UCL
Senescence-associated heterochromatin focus assembly Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm
Involvement in disease
The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.
Melanoma, cutaneous malignant 2 (CMM2): A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC): An inherited cancer predisposition syndrome characterized by an increased risk of developing malignant melanoma and/or pancreatic cancer. Mutation carriers within families may develop either or both types of cancer.
Melanoma-astrocytoma syndrome (MASTS): Characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma.
PTM
Phosphorylation seems to increase interaction with CDK4.
More Infomation

Xu, J., Li, N., Deng, W., & Luo, S. (2021). Discovering the mechanism and involvement of the methylation of cyclin-dependent kinase inhibitor 2A (CDKN2A) gene and its special locus region in gastric cancer. Bioengineered, 12(1), 1286-1298.

Salmani, T., Ghaderian, S. M. H., Hajiesmaeili, M., Mirghaed, O. R., Rakhshan, A., Nasiri, M. J., & Mohammadi, M. (2020). Cyclin dependent kinase inhibitor 2A and miR-671-5p expression profile in Iranian glioblastoma multiforme. Gene Reports, 19, 100620.

Althakfi, W., Gazzo, S., Blanchet, M., Isaac, S., Piaton, E., Villeneuve, L., ... & Brevet, M. (2020). The value of BRCA‐1‐associated protein 1 expression and cyclin‐dependent kinase inhibitor 2A deletion to distinguish peritoneal malignant mesothelioma from peritoneal location of carcinoma in effusion cytology specimens. Cytopathology, 31(1), 5-11.

Seifi, S., Pouya, F., Rahmani, M., Mehramiz, M., Rastgar‐Moghadam, A., Gharib, M., ... & Avan, A. (2020). Association of cyclin‐dependent kinase inhibitor 2A/B with increased risk of developing breast cancer. Journal of cellular physiology, 235(6), 5141-5145.

Zhang, H. H., Mao, J. S., & Hu, W. F. (2019). Functional genetic single-nucleotide polymorphisms (SNPs) in cyclin-dependent kinase inhibitor 2A/B (CDKN2A/B) locus are associated with risk and prognosis of osteosarcoma in chinese populations. Medical science monitor: international medical journal of experimental and clinical research, 25, 1307.

Zhang, W., Kuang, P., & Liu, T. (2019). Prognostic significance of CDKN2A/B deletions in acute lymphoblastic leukaemia: a meta-analysis. Annals of medicine, 51(1), 28-40.

Agarwal, M., Bakhshi, S., Dwivedi, S. N., Kabra, M., Shukla, R., & Seth, R. (2018). Cyclin dependent kinase inhibitor 2A/B gene deletions are markers of poor prognosis in Indian children with acute lymphoblastic leukemia. Pediatric blood & cancer, 65(6), e27001.

O'Hara, S. P., Splinter, P. L., Trussoni, C. E., Pisarello, M. J. L., Loarca, L., Splinter, N. S., ... & LaRusso, N. F. (2017). ETS proto-oncogene 1 transcriptionally up-regulates the cholangiocyte senescence-associated protein cyclin-dependent kinase inhibitor 2A. Journal of Biological Chemistry, 292(12), 4833-4846.

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For research use only. Not intended for any clinical use.

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