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Mouse Anti-CDCA8 Recombinant Antibody (CBFYC-0346) (CBMAB-C0385-FY)


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Published Data
CBMAB-C0385-FY-1
Mouse Anti-CDCA8 Recombinant Antibody (CBFYC-0346) (CBMAB-C0385-FY) in WB
The efficiency of transfection in Huh7 cell line were confirmed by qRT-PCR and Western blot. ...View More
Chen, E., He, Y., Jiang, J., Yi, J., Zou, Z., Song, Q., ... & Zhang, J. (2023). CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway. Experimental Hematology & Oncology, 12(1), 9.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-0346
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 46-86 mapping near the N-terminus of Borealin of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cell Division Cycle Associated 8
Introduction
CDCA8 (Cell Division Cycle Associated 8) is a Protein Coding gene. Diseases associated with CDCA8 include Uterine Corpus Endometrial Carcinoma and Uterine Corpus Cancer. Among its related pathways are SUMOylation and Metabolism of proteins.
Entrez Gene ID
55143
UniProt ID
Q53HL2
Alternative Names
Cell Division Cycle Associated 8; Pluripotent Embryonic Stem Cell-Related Gene 3 Protein; Cell Division Cycle-Associated Protein 8; Borealin; HDasra-B; Dasra-B
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Biological Process
Cell division Source: UniProtKB-KW
Chromosome organization Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Mitotic sister chromatid segregation Source: GO_Central
Mitotic spindle organization Source: Reactome
Cellular Location
Nucleolus; Cytoplasm; Spindle; Centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
PTM
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
More Infomation

Cui, X. H., Peng, Q. J., Li, R. Z., Lyu, X. J., Zhu, C. F., & Qin, X. H. (2021). Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma. Journal of Cellular and Molecular Medicine.

Li, W. X., Zhou, R. Q., Liu, Y., & Zhang, G. Y. (2021). Expression and clinical relevance of cell division cycle associated 8 (CDCA8) gene in endometrial cancer patients. European Journal of Gynaecological Oncology, 42(2), 278-285.

Li, B., Liu, B., Zhang, X., Liu, H., & He, L. (2020). KIF18B promotes the proliferation of pancreatic ductal adenocarcinoma via activating the expression of CDCA8. Journal of cellular physiology, 235(5), 4227-4238.

Gao, X., Wen, X., He, H., Zheng, L., Yang, Y., Yang, J., ... & Zhang, S. (2020). Knockdown of CDCA8 inhibits the proliferation and enhances the apoptosis of bladder cancer cells. PeerJ, 8, e9078.

Yu, D., Shi, L., Bu, Y., & Li, W. (2019). Cell division cycle associated 8 is a key regulator of tamoxifen resistance in breast cancer. Journal of breast cancer, 22(2), 237-247.

Ci, C., Tang, B., Lyu, D., Liu, W., Qiang, D., Ji, X., ... & Ding, W. (2019). Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis. International journal of molecular medicine, 43(1), 404-412.

Bu, Y., Shi, L., Yu, D., Liang, Z., & Li, W. (2019). CDCA8 is a key mediator of estrogen-stimulated cell proliferation in breast cancer cells. Gene, 703, 1-6.

Bi, Y., Chen, S., Jiang, J., Yao, J., Wang, G., Zhou, Q., & Li, S. (2018). CDCA8 expression and its clinical relevance in patients with bladder cancer. Medicine, 97(34).

Phan, N. N., Wang, C. Y., Li, K. L., Chen, C. F., Chiao, C. C., Yu, H. G., ... & Lin, Y. C. (2018). Distinct expression of CDCA3, CDCA5, and CDCA8 leads to shorter relapse free survival in breast cancer patient. Oncotarget, 9(6), 6977.

Jeon, T. W., Ko, M. J., Seo, Y. R., Baik, I. H., Hwang, I., Ryoo, H. M., ... & Lee, Y. H. (2017). Knockdown of cell division cycle-associated 8 (CDCA8) suppresses hepatocellular carcinoma growth via the upregulation of tumor suppressor ATF3.

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For research use only. Not intended for any clinical use.

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