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Mouse Anti-CD46 Recombinant Antibody (CBFYC-0242) (CBMAB-C0271-FY)


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Published Data
CBMAB-C0271-FY-1
Mouse Anti-CD46 Recombinant Antibody (CBFYC-0242) (CBMAB-C0271-FY) in FC
Cyt-1, Cyt-2, and IL-10 mRNA quantification after in vitro anti-CD3/CD46 MoAb stimulation. mRNA levels of Cyt-1 (a), Cyt-2 (b), Cyt-2 and Cyt-1 ratio (c), and IL-10 mRNA levels (d) in CD4+ cells pre- and post- in vitro stimulation. CD4+ cells obtained from IFN-β-treated patients before therapy initiation (T0) and then after 12 (T12) and 24 (T24) months of therapy and healthy controls (Ctrl) and stimulated with anti-CD3 and anti-CD46 MoAbs. Results are calculated as normalization ratio (NR), relative to a common standard sample. Estimated means are shown, connected by continuous lines, along with error bars representing the 95% confidence interval. Other lines indicate statistical significance. ...View More
Chiarini, M., Capra, R., Serana, F., Bertoli, D., Sottini, A., Giustini, V., ... & SURROGATE Study Group. (2020). Simultaneous quantification of natural and inducible regulatory T-cell subsets during interferon-β therapy of multiple sclerosis patients. Journal of Translational Medicine, 18, 1-11. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0271-FY-2
Mouse Anti-CD46 Recombinant Antibody (CBFYC-0242) (CBMAB-C0271-FY) in IP
A specific HHV-6-associated protein co-immunoprecipitates with CD46. A, immunoprecipitation by an anti-CD46 mAb (J4.48) of proteins from a lysate of [35S]methionine-labeled HHV-6-infected HSB-2 cells and unlabeled NIH 3T3 cells infected with a vaccinia virus expressing human CD46 (vCB48). Uninfected HSB-2 cells and NIH 3T3 cells infected with a control vaccinia virus (WR) served as negative controls. B, immunoprecipitation by mAbs against influenza HA (HA.11) or human CD46 (J4.48) of proteins from a lysate of [35S]methionine-labeled HHV-6-infected HSB-2 cells and unlabeled NIH 3T3 cells expressing human CD46. The immunoprecipitated proteins were analyzed by SDS-PAGE and autoradiography. ...View More
Santoro, F., Greenstone, H. L., Insinga, A., Liszewski, M. K., Atkinson, J. P., Lusso, P., & Berger, E. A. (2003). Interaction of glycoprotein H of human herpesvirus 6 with the cellular receptor CD46. Journal of Biological Chemistry, 278(28), 25964-25969. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0271-FY-3
Mouse Anti-CD46 Recombinant Antibody (CBFYC-0242) (CBMAB-C0271-FY) in IP
Immunoprecipitation was performed with the anti-CD46 mAb J4.48, and immunoblotting was performed with rabbit anti-gH antiserum. The NIH 3T3 cells either expressed (+) or did not express (-) CD46. ...View More
Santoro, F., Greenstone, H. L., Insinga, A., Liszewski, M. K., Atkinson, J. P., Lusso, P., & Berger, E. A. (2003). Interaction of glycoprotein H of human herpesvirus 6 with the cellular receptor CD46. Journal of Biological Chemistry, 278(28), 25964-25969. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0242
Antibody Isotype
IgG2a, κ
Application
WB, IP, FC, IF

Basic Information

Immunogen
Human PBL and EBV transformed LCL.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD46 Molecule
Introduction
CD46 (CD46 Molecule) is a Protein Coding gene. Diseases associated with CD46 include Hemolytic Uremic Syndrome, Atypical 2 and Measles. Among its related pathways are Creation of C4 and C2 activators and Complement and coagulation cascades. Gene Ontology (GO) annotations related to this gene include receptor activity and enzyme inhibitor activity. An important paralog of this gene is C4BPA.
Entrez Gene ID
4179
UniProt ID
P15529
Function
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.
(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.
(Microbial infection) Acts as a receptor for cultured Measles virus.
(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.
(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).
Biological Process
Adaptive immune response Source: UniProtKB
Complement activation, classical pathway Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of gene expression Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of memory T cell differentiation Source: UniProtKB
Positive regulation of regulatory T cell differentiation Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transforming growth factor beta production Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of Notch signaling pathway Source: UniProtKB
Sequestering of extracellular ligand from receptor Source: UniProtKB
Single fertilization Source: UniProtKB-KW
T cell mediated immunity Source: UniProtKB
Cellular Location
Acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
Involvement in disease
Hemolytic uremic syndrome atypical 2 (AHUS2): Disease susceptibility is associated with variants affecting the gene represented in this entry. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease.
Topology
Extracellular: 35-343
Helical: 344-366
Cytoplasmic: 367-392
PTM
N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.
More Infomation

Wang, S., Li, J., Hua, J., Su, Y., Beckford-Vera, D. R., Zhao, W., ... & He, J. (2021). Molecular imaging of prostate cancer targeting CD46 using immunoPET. Clinical Cancer Research, 27(5), 1305-1315.

Wang, S., Su, Y., Escobar, B. P., Steri, V., Hann, B., Tang, R., ... & Flavell, R. (2021). Molecular Imaging of Multiple Myeloma Targeting CD46 Using ImmunoPET.

Muñoz-Alía, M. Á., Nace, R. A., Tischer, A., Zhang, L., Bah, E. S., Auton, M., & Russell, S. J. (2021). MeV-Stealth: A CD46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum. PLoS pathogens, 17(2), e1009283.

Schack, V. R., Rossen, L. S., Ekebjærg, C. C., Thuesen, K. K. H., Bundgaard, B., & Höllsberg, P. (2021). The tetraspanin protein CD9 modulates infection with human herpesvirus 6A and 6B in a CD46-dependent manner. Journal of Virology, 95(8), e02259-20.

Feng, Y., Yi, C., Liu, X., Qu, L., Su, W., Shu, T., ... & Feng, L. (2020). Human desmoglein-2 and human CD46 mediate human adenovirus type 55 infection, but human desmoglein-2 plays the major roles. Journal of virology, 94(17), e00747-20.

Riedel, C., Chen, H. W., Reichart, U., Lamp, B., Laketa, V., & Rümenapf, T. (2020). Real time analysis of bovine viral diarrhea virus (BVDV) infection and its dependence on bovine CD46. Viruses, 12(1), 116.

Sajjadi, S., Shirode, A., Vaidya, S. R., & Cherian, S. S. (2019). Molecular mechanism by which residues at position 481 and 546 of measles virus hemagglutinin protein define CD46 receptor binding using a molecular docking approach. Computational biology and chemistry, 80, 384-389.

Liszewski, M. K., & Kemper, C. (2019). Complement in motion: the evolution of CD46 from a complement regulator to an orchestrator of normal cell physiology. Journal of immunology (Baltimore, Md.: 1950), 203(1), 3.

Ni Choileain, S., Hay, J., Thomas, J., Williams, A., Vermeren, M. M., Benezech, C., ... & Astier, A. L. (2017). TCR-stimulated changes in cell surface CD46 expression generate type 1 regulatory T cells. Science signaling, 10(502), eaah6163.

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For research use only. Not intended for any clinical use.

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