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Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CBMAB-C0033-FY)


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Published Data
CBMAB-C0033-FY-1
Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CBMAB-C0033-FY) in WB
Western blot analysis of HER2.z, HER2.CD28.z, HER2.41BB.z and HER2.CD28.41BB.z CAR T cells. The membranes were probed for anti-human CD3ζ. ...View More
Mezősi-Csaplár, M., Szöőr, Á., & Vereb, G. (2023). CD28 and 41BB costimulatory domains alone or in combination differentially influence cell surface dynamics and organization of chimeric antigen receptors and early activation of CAR T cells. Cancers, 15(12), 3081. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0033-FY-2
Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CBMAB-C0033-FY) in IF
Representative microscopic images following 15 min of effector-tumor co-culturing. ...View More
Mezősi-Csaplár, M., Szöőr, Á., & Vereb, G. (2023). CD28 and 41BB costimulatory domains alone or in combination differentially influence cell surface dynamics and organization of chimeric antigen receptors and early activation of CAR T cells. Cancers, 15(12), 3081. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0033-FY-3
Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CBMAB-C0033-FY) in WB
Analysis of SLP-76 and CD3ζ phosphorylation upon co-expression with SYK, ZAP70, Fyn, or Csk. ...View More
Fasbender, F., Claus, M., Wingert, S., Sandusky, M., & Watzl, C. (2017). Differential requirements for Src-family kinases in SYK or ZAP70-mediated SLP-76 phosphorylation in lymphocytes. Frontiers in immunology, 8, 789. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0026
Antibody Isotype
IgG1, κ
Application
WB, IP, ELISA, FC

Basic Information

Immunogen
Amino acids 52-163 mapping within the cytoplasmic domain of CD3-ζ precursor of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD247 Molecule
Introduction
CD247 (CD247 Molecule) is a Protein Coding gene. Diseases associated with CD247 include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are G-protein signaling N-RAS regulation pathway and Role of phospholipids in phagocytosis. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transmembrane signaling receptor activity.
Entrez Gene ID
919
UniProt ID
P20963
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Topology
Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
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For research use only. Not intended for any clinical use.

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