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Rat Anti-CD180 Recombinant Antibody (RP/14) (CBMAB-R0468-CN)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
RP/14
Antibody Isotype
IgG2a
Application
IP, IF, FC, ELISA

Basic Information

Immunogen
Transfected cells expressing RP105 of mouse origin.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml

Target

Full Name
CD180 Molecule
Introduction
CD180 (CD180 Molecule) is a Protein Coding gene. Among its related pathways are Innate Immune System and Activated TLR4 signalling. An important paralog of this gene is TLR4.
Entrez Gene ID
17079
UniProt ID
Q62192
Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity).
Biological Process
B cell proliferation involved in immune response Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
Cellular Location
Cell membrane
Topology
Extracellular: 24-626
Helical: 627-650
Cytoplasmic: 651-661
More Infomation

Hayden, Z., Erdő-Bonyár, S., Bóné, B., Balázs, N., Bodó, K., Illes, Z., ... & Simon, D. (2021). Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders. Journal of Immunology Research, 2021.

Miguet, L., Mayeur-Rousse, C., Eischen, A., Galoisy, A. C., Rolland, D., Gervais, C., ... & Mauvieux, L. (2021). The Contribution of CD148, CD180 and CD200 Combination in the Diagnosis of Chronic B-Cell Lymphoproliferative Disorders. Blood, 138(Supplement 1), 3520-3520.

Edwards, K., Volpi, E., Lydyard, P., Zaitseva, K., Sayed, U., Krysov, S., ... & Porakishvili, N. (2021). Expression patterns of CD180 in the lymph nodes of patients with chronic lymphocytic leukaemia. British Journal of Haematology, 195(2), e131-e134.

Dong, G., Yao, X., Yan, F., Zhang, H., Zhu, Y., Yang, Y., ... & Xiong, H. (2019). Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(3), 535-546.

Roe, K., Shu, G. L., Draves, K. E., Giordano, D., Pepper, M., & Clark, E. A. (2019). Targeting antigens to CD180 but not CD40 programs immature and mature B cell subsets to become efficient APCs. The Journal of Immunology, 203(7), 1715-1729.

Kikuchi, J., Kuroda, Y., Koyama, D., Osada, N., Izumi, T., Yasui, H., ... & Furukawa, Y. (2018). Myeloma cells are activated in bone marrow microenvironment by the CD180/MD-1 complex, which senses lipopolysaccharide. Cancer research, 78(7), 1766-1778.

Carreras-González, A., Navasa, N., Martín-Ruiz, I., Lavín, J. L., Azkargorta, M., Atondo, E., ... & Anguita, J. (2018). A multi-omic analysis reveals the regulatory role of CD180 during the response of macrophages to Borrelia burgdorferi. Emerging microbes & infections, 7(1), 1-13.

Yang, Y., Wang, C., Cheng, P., Zhang, X., Li, X., Hu, Y., ... & Xiong, H. (2018). CD180 Ligation inhibits TLR7-and TLR9-mediated activation of macrophages and dendritic cells through the Lyn-SHP-1/2 axis in murine lupus. Frontiers in immunology, 9, 2643.

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For research use only. Not intended for any clinical use.

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