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Mouse Anti-CCT4 Recombinant Antibody (CBYJT-1072) (CBMAB-T0082-YJ)


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Published Data
CBMAB-T0082-YJ-1
Mouse Anti-CCT4 Recombinant Antibody (CBYJT-1072) (CBMAB-T0082-YJ) in WB
Reverse phase chromatogram of recombinant hTRiC, with peaks labelled by the predominant eluting subunit(s), as determined by Western blots. ...View More
Collier, M. P., Moreira, K. B., Li, K. H., Chen, Y. C., Itzhak, D., Samant, R., ... & Frydman, J. (2021). Native mass spectrometry analyses of chaperonin complex TRiC/CCT reveal subunit N-terminal processing and re-association patterns. Scientific Reports, 11(1), 13084. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-T0082-YJ-2
Mouse Anti-CCT4 Recombinant Antibody (CBYJT-1072) (CBMAB-T0082-YJ) in WB
GFP pulldowns confirm the interaction between CSA and TRiC subunits CCT4 and CCT5. GFP or CSA-GFP was pulled down from CS3BE-SV40 cells. ...View More
Pines, A., Dijk, M., Makowski, M., Meulenbroek, E. M., Vrouwe, M. G., Van Der Weegen, Y., ... & van Attikum, H. (2018). TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nature Communications, 9(1), 1040. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJT-1072
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB, IHC-P

Basic Information

Immunogen
Amino acids 176-400 mapping within an internal region of TCP-1 δ of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Chaperonin Containing TCP1 Subunit 4
Introduction
CCT4 (Chaperonin Containing TCP1 Subunit 4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex it may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Entrez Gene ID
Human10575
Mouse12464
Cattle613336
UniProt ID
HumanP50991
MouseP80315
CattleF1N0E5
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444).
The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444).
As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638).
The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Biological Process
Binding of sperm to zona pellucida Source: Ensembl
Positive regulation of establishment of protein localization to telomere Source: BHF-UCL
Positive regulation of protein localization to Cajal body Source: BHF-UCL
Positive regulation of telomerase activity Source: BHF-UCL
Positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein folding Source: FlyBase
Protein stabilization Source: BHF-UCL
scaRNA localization to Cajal body Source: BHF-UCL
Toxin transport Source: Ensembl
Cellular Location
Cytoplasm; Centrosome; Cilium basal body; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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For research use only. Not intended for any clinical use.

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