Mouse Anti-CCDC134 Recombinant Antibody (CBFYC-0909) (CBMAB-C0965-FY)

Name: Mouse Anti-CCDC134 Recombinant Antibody (CBFYC-0909)
SKU: CBMAB-C0965-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYC-0909

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 21-153 mapping within an internal region of CCDC134 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Coiled-Coil Domain Containing 134

Introduction

CCDC134 (Coiled-Coil Domain Containing 134) is a Protein Coding gene.

Entrez Gene ID

79879

UniProt ID

Q9H6E4

Alternative Names

Coiled-Coil Domain Containing 134

Function

In extracellular secreted form, promotes proliferation and activation of CD8+ T cells, suggesting a cytokine-like function (PubMed:25125657).
Enhances cytotoxic anti-tumor activity of CD8+ T cells (PubMed:25125657).
May inhibit ERK and JNK signaling activity (PubMed:18087676, PubMed:23070808).
May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed:23070808).
In the nucleus, enhances stability of the PCAF histone acetyltransferase (HAT) complex member TADA2A and thus promotes PCAF-mediated H3K14 and H4K8 HAT activity. May inhibit TADA2A-mediated TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity. May also promote TADA2A-mediated XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage.

Biological Process

Angiogenesis Source: Ensembl
Embryonic hemopoiesis Source: Ensembl
Embryonic liver development Source: Ensembl
Placenta development Source: Ensembl
Ventricular system development Source: Ensembl

Cellular Location

Endoplasmic reticulum; Cytoplasm; Secreted; Nucleus. Accumulates in the nucleus in response to UV irradiation (PubMed:22644376).

PTM

O-glycosylated, with additional sialic acid modifications.

More Infomation

References

Yin, S., Liao, Q., Wang, Y., Shi, Q., Xia, P., Yi, M., & Huang, J. (2021). Ccdc134 deficiency impairs cerebellar development and motor coordination. Genes, Brain and Behavior, 20(7), e12763.

Holick, M. F., Shirvani, A., & Charoenngam, N. (2021). Fetal Fractures in an Infant with Maternal Ehlers-Danlos Syndrome, CCDC134 Pathogenic Mutation and a Negative Genetic Test for Osteogenesis Imperfecta. Children, 8(6), 512.

Dubail, J., Brunelle, P., Baujat, G., Huber, C., Doyard, M., Michot, C., ... & Cormier‐Daire, V. (2020). Homozygous loss‐of‐function mutations in CCDC134 are responsible for a severe form of osteogenesis imperfecta. Journal of Bone and Mineral Research, 35(8), 1470-1480.

Xia, P., Gong, X., Xiao, L., Wang, Y., Zhang, T., Liao, Q., ... & Huang, J. (2018). CCDC134 ameliorated experimental autoimmune encephalomyelitis by suppressing Th1 and Th17 cells. Brain, behavior, and immunity, 71, 158-168.

Yu, B., Zhang, T., Xia, P., Gong, X., Qiu, X., & Huang, J. (2018). CCDC134 serves a crucial role in embryonic development. International journal of molecular medicine, 41(1), 381-390.

Xia, P., Zhang, T., Gong, X., Xiao, L., Liao, Q., Qiu, X., & Huang, J. (2017). Amelioration of adjuvant-induced arthritis in CCDC134-overexpressing transgenic mice. Biochemical and biophysical research communications, 490(2), 111-116.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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