Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CBLC Recombinant Antibody (CBFYC-0889) (CBMAB-C0944-FY)


See all CBLC antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0889
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 438-474 of Cbl-3 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cas-Br-M (murine) ecotropic retroviral transforming sequence c
Introduction
This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Entrez Gene ID
23624
UniProt ID
Q9ULV8
Alternative Names
Cbl Proto-Oncogene C; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence C; Cas-Br-M (Murine) Ectropic Retroviral Transforming Sequence C; Cbl Proto-Oncogene C, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase CBL-C; Signal Transduction Protein CBL-C; SH3-Binding Protein CBL-3; SH3-Binding Protein CBL-C; RING Finger Protein 57
Function
Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.
Biological Process
Cell surface receptor signaling pathway Source: InterPro
Negative regulation of epidermal growth factor-activated receptor activity Source: BHF-UCL
Negative regulation of epidermal growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of MAP kinase activity Source: BHF-UCL
Protein ubiquitination Source: BHF-UCL
Signal transduction Source: GO_Central
Ubiquitin-dependent protein catabolic process Source: BHF-UCL
Cellular Location
Plasma membrane; Membrane raft
PTM
Phosphorylated on multiple tyrosine residues by SRC. Isoform 1, but not isoform 2, is phosphorylated on tyrosines by EGFR.
Autoubiquitinated when phosphorylated at Tyr-341, enhanced by SRC; suggesting proteasomal degradation.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CBLC Recombinant Antibody (CBFYC-0889)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry