Mouse Anti-CAVIN3 Recombinant Antibody (8D3) (CBMAB-P0142-YC)

Name: Mouse Anti-CAVIN3 Recombinant Antibody (8D3)
SKU: CBMAB-P0142-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

8D3

Antibody Isotype

IgG1, κ

Application

ELISA, WB, IP

Basic Information

Immunogen

Amino acids 161-261 of PRKCDBP of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 161-261

Target

Full Name

Caveolae Associated Protein 3

Introduction

CAVIN3 was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein.

Entrez Gene ID

112464

UniProt ID

Q969G5

Alternative Names

CAVIN3; cavin-3; SRBC; HSRBC

Function

Regulates the traffic and/or budding of caveolae (PubMed:19262564).
Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in smooth muscle but not in the lung and heart endothelial cells. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by promoting the rapid release of caveolae from the cell surface. Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2 (By similarity).

Biological Process

Circadian regulation of gene expression Source: UniProtKB

Cellular Location

Cytoplasm; Cytosol; Caveola. Localizes in the caveolae in a caveolin-dependent manner.

PTM

In vitro, phosphorylated by PRKCD.

More Infomation

References

McMahon, K. A., Stroud, D. A., Gambin, Y., Tillu, V., Bastiani, M., Sierecki, E., ... & Parton, R. G. (2021). Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response. Elife, 10, e61407.

Park, P. J., & Kim, S. T. (2020). Caveolae-Associated Protein 3 (Cavin-3) Influences Adipogenesis via TACE-Mediated Pref-1 Shedding. International journal of molecular sciences, 21(14), 5000.

Yamaguchi, T., Hayashi, M., Ida, L., Yamamoto, M., Lu, C., Kajino, T., ... & Takahashi, T. (2019). ROR1-CAVIN3 interaction required for caveolae-dependent endocytosis and pro-survival signaling in lung adenocarcinoma. Oncogene, 38(26), 5142-5157.

Zhu, B., Swärd, K., Ekman, M., Uvelius, B., & Rippe, C. (2017). Cavin‐3 deficiency reduces caveolae abundance in vascular and urinary bladder smooth muscle cells. The FASEB Journal, 31, 1015-23.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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