Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CASP5 Recombinant Antibody (C6948) (CBMAB-C6948-LY)


See all CASP5 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
C6948
Antibody Isotype
IgM, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 120-153 at the N-terminus of caspase-5 p20 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Caspase 5
Introduction
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Entrez Gene ID
838
UniProt ID
P51878
Function
Thiol protease that acts as a mediator of programmed cell death (PubMed:29898893, PubMed:28314590).
Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893).
During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).
Biological Process
Apoptotic process Source: ProtInc
Cellular response to mechanical stimulus Source: UniProtKB
Execution phase of apoptosis Source: GOC
Proteolysis Source: ProtInc
Regulation of apoptotic process Source: InterPro
Regulation of inflammatory response Source: GO_Central
Substantia nigra development Source: UniProtKB
Cellular Location
NLRP1 inflammasome complex; Cytoplasm
PTM
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CASP5 Recombinant Antibody (C6948)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry